Anti-CDC20 Antibody (CAB15656)
- SKU:
- CAB15656
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-CDC20 Antibody |
| 抗体コード: | CAB15656 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC20 (NP_001246.2). |
| 申し込み: | WB IHC IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | LO2, HT-29, A-431 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC20 (NP_001246.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAQF AFES DLHS LLQL DAPI PNAP PARW QRKA KEAA GPAP SPMR AANR SHSA GRTP GRTP GKSS SKVQ TTPS KPGG DRYI PHRS AAQM EVAS FLLS KENQ PENS QTPT KKEH QKAW ALNL NGFD VEEA KILR LSGK PQNA PEGY QNRL KVLY SQKA TPGS SRKT CRYI PSLP DRIL DAPE IRND YYLN LVDW SSGN VLAV ALDN SVYL WSAS SGDI LQLL QMEQ PGEY ISSV AWIK EGNY LAVG TSSA EVQL WDVQ QQKR LRNM TSHS AR |
| 遺伝子ID: | 991 |
| Uniprot: | Q12834 |
| セルラーロケーション: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole |
| 計算された分子量: | 54kDa |
| 観察された分子量: | 55kDa |
| 同義語: | CDC20, CDC20A, bA276H19.3, p55CDC |
| バックグラウンド: | CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. |
| UniProt Protein Function: | CDC20: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with isoform 1 of NEK2. Belongs to the WD repeat CDC20/Fizzy family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Cell cycle regulation Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: anaphase-promoting complex; cytoplasm; cytosol; nucleoplasm; nucleus; spindle Molecular Function:enzyme binding; protein binding; protein C-terminus binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of synaptic plasticity; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; sister chromatid cohesion |
| NCBI Summary: | CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12834 |
| NCBI GenInfo Identifier: | 37537762 |
| NCBI Gene ID: | 991 |
| NCBI Accession: | Q12834.2 |
| UniProt Secondary Accession: | Q12834,Q5JUY4, Q9BW56, Q9UQI9, B2R6Z6, D3DPJ1, |
| UniProt Related Accession: | Q12834 |
| Molecular Weight: | 54,723 Da |
| NCBI Full Name: | Cell division cycle protein 20 homolog |
| NCBI Synonym Full Names: | cell division cycle 20 |
| NCBI Official Symbol: | CDC20 |
| NCBI Official Synonym Symbols: | CDC20A; p55CDC; bA276H19.3 |
| NCBI Protein Information: | cell division cycle protein 20 homolog |
| UniProt Protein Name: | Cell division cycle protein 20 homolog |
| UniProt Synonym Protein Names: | p55CDC |
| Protein Family: | Cell division cycle protein |
| UniProt Gene Name: | CDC20 |
| UniProt Entry Name: | CDC20_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDC20 antibody (CAB15656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded Rat ovary using CDC20 antibody (CAB15656) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat spleen using CDC20 antibody (CAB15656) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human thyroid cancer using CDC20 antibody (CAB15656) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of LO2 cells using 3ug CDC20 antibody (CAB15656). Western blot was performed from the immunoprecipitate using CDC20 antibody (CAB15656) at a dilition of 1:1000. |
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