Anti-CDC16 Antibody (CAB7197)
- SKU:
- CAB7197
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-CDC16 Antibody |
| 抗体コード: | CAB7197 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 371-620 of human CDC16 (NP_003894.3). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Jurkat, HepG2, HeLa, A-549, Mouse brain, Mouse ovary, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 371-620 of human CDC16 (NP_003894.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MLYI GLEY GLTN NSKL AERF FSQA LSIA PEDP FVMH EVGV VAFQ NGEW KTAE KWFL DALE KIKA IGNE VTVD KWEP LLNN LGHV CRKL KKYA EALD YHRQ ALVL IPQN ASTY SAIG YIHS LMGN FENA VDYF HTAL GLRR DDTF SVTM LGHC IEMY IGDS EAYI GADI KDKL KCYD FDVH TMKT LKNI ISPP WDFR EFEV EKQT AEET GLTP LETS RKTP DSRP SLEE TFEI EMNE SDMM LETS MSDH ST |
| 遺伝子ID: | 8881 |
| Uniprot: | Q13042 |
| セルラーロケーション: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle |
| 計算された分子量: | 60kDa/65kDa/71kDa |
| 観察された分子量: | 72kDa |
| 同義語: | CDC16, ANAPC6, APC6, CDC16Hs, CUT9 |
| バックグラウンド: | The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. |
| UniProt Protein Function: | CDC16: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C is composed of at least 12 subunits. Interacts with PPP5C and CDC20. Belongs to the APC6/CDC16 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 13q34 Cellular Component: nucleoplasm; centrosome; anaphase-promoting complex; spindle microtubule; cytoplasm; spindle; cytosol Molecular Function:protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell proliferation; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; regulation of mitosis; mitotic cell cycle |
| NCBI Summary: | This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13042 |
| NCBI GenInfo Identifier: | 37537763 |
| NCBI Gene ID: | 8881 |
| NCBI Accession: | Q13042.2 |
| UniProt Secondary Accession: | Q13042,Q5T8C8, Q7Z651, Q96AE6, Q9Y564, A2A365, |
| UniProt Related Accession: | Q13042 |
| Molecular Weight: | 620 |
| NCBI Full Name: | Cell division cycle protein 16 homolog |
| NCBI Synonym Full Names: | cell division cycle 16 |
| NCBI Official Symbol: | CDC16 |
| NCBI Official Synonym Symbols: | APC6; CUT9; ANAPC6 |
| NCBI Protein Information: | cell division cycle protein 16 homolog; CDC16Hs; cyclosome subunit 6; cell division cycle 16 homolog; anaphase-promoting complex subunit 6; anaphase-promoting complex, subunit 6 |
| UniProt Protein Name: | Cell division cycle protein 16 homolog |
| UniProt Synonym Protein Names: | Anaphase-promoting complex subunit 6; APC6; CDC16 homolog; CDC16Hs; Cyclosome subunit 6 |
| Protein Family: | Cell division control protein |
| UniProt Gene Name: | CDC16 |
| UniProt Entry Name: | CDC16_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDC16 antibody (CAB7197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using CDC16 antibody (CAB7197) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using CDC16 antibody (CAB7197) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using CDC16 antibody (CAB7197). Blue: DAPI for nuclear staining. |
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