Anti-CD99 Antibody (RACO0043)
- SKU:
- RACO0043
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | CD99 Antibody |
| Product SKU: | RACO0043 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC, FC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:500 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity). |
| シノニム: | CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD99, CD99, MIC2, MIC2X, MIC2Y |
 | IHC image of RACO0043 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Overlay histogram showing Jurkat cells stained with RACO0043 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. |
 | Western Blot. Positive WB detected in Jurkat whole cell lysate All lanes CD99 antibody at 0.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 28 KDa. Observed band size: 28 KDa. |
| UniProt Protein Function: | Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. |
| NCBI Summary: | The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016] |
| UniProt Code: | P14209 |
| NCBI GenInfo Identifier: | 119049 |
| NCBI Gene ID: | 4267 |
| NCBI Accession: | P14209.1 |
| UniProt Secondary Accession: | P14209,O00518, Q6ICV7, A6NIW1, |
| UniProt Related Accession: | P14209 |
| Molecular Weight: | 17,128 Da |
| NCBI Full Name: | CD99 antigen |
| NCBI Synonym Full Names: | CD99 molecule (Xg blood group) |
| NCBI Official Symbol: | CD99 |
| NCBI Official Synonym Symbols: | MIC2; HBA71; MIC2X; MIC2Y; MSK5X |
| NCBI Protein Information: | CD99 antigen |
| UniProt Protein Name: | CD99 antigen |
| UniProt Synonym Protein Names: | 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD_antigen: CD99 |
| Protein Family: | CD99 antigen |
| UniProt Gene Name: | CD99 |