Anti-CD8B Antibody (CAB3286)
- SKU:
- CAB3286
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-CD8B Antibody |
| 抗体コード: | CAB3286 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-170 of human CD8B (NP_757362.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, Mouse thymus, Mouse liver, Mouse kidney, Rat thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-170 of human CD8B (NP_757362.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LQQT PAYI KVQT NKMV MLSC EAKI SLSN MRIY WLRQ RQAP SSDS HHEF LALW DSAK GTIH GEEV EQEK IAVF RDAS RFIL NLTS VKPE DSGI YFCM IVGS PELT FGKG TQLS VVDF LPTT AQPT KKST LKKR VCRL PRPE TQKG PLCS P |
| 遺伝子ID: | 926 |
| Uniprot: | P10966 |
| セルラーロケーション: | Cell membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein |
| 計算された分子量: | 20kDa/22kDa/23kDa/24kDa/25kDa/27kDa |
| 観察された分子量: | 45kDa |
| 同義語: | CD8B, CD8B1, LEU2, LY3, LYT3, P37 |
| バックグラウンド: | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. |
| UniProt Protein Function: | CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 2p12 Cellular Component: T cell receptor complex; integral to plasma membrane; early endosome membrane; plasma membrane; extracellular region; external side of plasma membrane Molecular Function:protein binding; MHC class I protein binding; coreceptor activity Biological Process: regulation of immune response; T cell activation; viral reproduction; immune response; regulation of defense response to virus by virus; transmembrane receptor protein tyrosine kinase signaling pathway |
| NCBI Summary: | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010] |
| UniProt Code: | P10966 |
| NCBI GenInfo Identifier: | 116032 |
| NCBI Gene ID: | 926 |
| NCBI Accession: | P10966.1 |
| UniProt Secondary Accession: | P10966,P14860, P14861, Q15980, Q496E0, Q496E1, Q496E2 Q9UDB4, Q9UDB5, Q9UDB6, Q9UDB7, Q9UDB8, |
| UniProt Related Accession: | P10966 |
| Molecular Weight: | 210 |
| NCBI Full Name: | T-cell surface glycoprotein CD8 beta chain |
| NCBI Synonym Full Names: | CD8b molecule |
| NCBI Official Symbol: | CD8B |
| NCBI Official Synonym Symbols: | LY3; P37; LEU2; LYT3; CD8B1 |
| NCBI Protein Information: | T-cell surface glycoprotein CD8 beta chain; CD8 antigen, beta polypeptide 1 (p37); T lymphocyte surface glycoprotein beta chain |
| UniProt Protein Name: | T-cell surface glycoprotein CD8 beta chain |
| UniProt Synonym Protein Names: | CD_antigen: CD8b |
| Protein Family: | T-cell surface glycoprotein |
| UniProt Gene Name: | CD8B |
| UniProt Entry Name: | CD8B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD8B antibody (CAB3286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |