Anti-CD80 Antibody (RACO0309)
- SKU:
- RACO0309
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | CD80 Antibody |
| Product SKU: | RACO0309 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC, IP |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human CD80 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. |
| シノニム: | T-lymphocyte activation antigen CD80 (Activation B7-1 antigen) (BB1) (CTLA-4 counter-receptor B7.1) (B7) (CD antigen CD80), CD80, CD28LG CD28LG1 LAB7 |
 | IHC image of RACO0309 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Immunoprecipitating CD80 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of RACO0309 in Raji whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0309(2µg)+ Raji whole cell lysate (500µg). Lane 3: Raji whole cell lysate (10µg). |
 | Western Blot. Positive WB detected in Raji whole cell lysate All lanes CD80 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 34, 30, 19 kDa. Observed band size: 60 kDa. |
| UniProt Protein Function: | Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. |
| NCBI Summary: | The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P33681 |
| NCBI GenInfo Identifier: | 461606 |
| NCBI Gene ID: | 941 |
| NCBI Accession: | P33681.1 |
| UniProt Secondary Accession: | P33681,Q5DTA9, Q5DTB0, |
| UniProt Related Accession: | P33681 |
| Molecular Weight: | 18,761 Da |
| NCBI Full Name: | T-lymphocyte activation antigen CD80 |
| NCBI Synonym Full Names: | CD80 molecule |
| NCBI Official Symbol: | CD80 |
| NCBI Official Synonym Symbols: | B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1 |
| NCBI Protein Information: | T-lymphocyte activation antigen CD80 |
| UniProt Protein Name: | T-lymphocyte activation antigen CD80 |
| UniProt Synonym Protein Names: | Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD_antigen: CD80 |
| Protein Family: | T-lymphocyte activation antigen |
| UniProt Gene Name: | CD80 |