Anti-CD47[KO Validated] Antibody (CAB11382)
![Anti-CD47[KO Validated] Antibody (CAB11382)](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/25616/25464/anti-cd47ko-validated-antibody-cab11382__85560__24036.1645806304.jpg?c=1 "Anti-CD47[KO Validated] Antibody (CAB11382)")
- SKU:
- CAB11382
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Biology
![Anti-CD47[KO Validated] Antibody (CAB11382)](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/25616/25464/anti-cd47ko-validated-antibody-cab11382__85560__24036.1645806304.jpg?c=1&imbypass=on "Anti-CD47[KO Validated] Antibody (CAB11382)")
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Description
| 抗体名: | Anti-CD47[KO Validated] Antibody |
| 抗体コード: | CAB11382 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthesized peptide derived from human CD47 |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, U2OS, MCF7, Mouse brain, Mouse spleen, Rat brain, Rat kidney |
| 免疫原: | A synthesized peptide derived from human CD47 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 961 |
| Uniprot: | Q08722 |
| セルラーロケーション: | Cell membrane, Multi-pass membrane protein |
| 計算された分子量: | 55kDa |
| 観察された分子量: | 50KDa |
![Anti-CD47[KO Validated] Antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/y/946/A11382_1__73206.jpg) | Western blot analysis of extracts of various cell lines, using CD47 Rabbit mAb (CAB11382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
![Anti-CD47[KO Validated] Antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/d/214/A11382_2__36930.jpg) | Western blot analysis of extracts of various cell lines, using CD47 Rabbit mAb (CAB11382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
![Anti-CD47[KO Validated] Antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/x/280/A11382_3__08324.jpg) | Western blot analysis of extracts from normal (control) and CD47 knockout (KO) MCF7 cells and U2OS cells, using CD47 antibody (CAB11382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
| 同義語: | IAP, MER6, OA3 |
| バックグラウンド: | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Protein Function: | CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3q13.1-q13.2 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response |
| NCBI Summary: | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Code: | Q08722 |
| NCBI GenInfo Identifier: | 1171879 |
| NCBI Gene ID: | 961 |
| NCBI Accession: | Q08722.1 |
| UniProt Related Accession: | Q08722 |
| Molecular Weight: | |
| NCBI Full Name: | Leukocyte surface antigen CD47 |
| NCBI Synonym Full Names: | CD47 molecule |
| NCBI Official Symbol: | CD47 |
| NCBI Official Synonym Symbols: | IAP; OA3; MER6 |
| NCBI Protein Information: | leukocyte surface antigen CD47 |
| UniProt Protein Name: | Leukocyte surface antigen CD47 |
| UniProt Synonym Protein Names: | Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47 |
| Protein Family: | IgE-binding protein |
| UniProt Gene Name: | CD47 |
| UniProt Entry Name: | CD47_HUMAN |