Anti-CD47 Antibody (RACO0401)
- SKU:
- RACO0401
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | CD47 Antibody |
| Product SKU: | RACO0401 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human CD47 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. |
| シノニム: | Leukocyte surface antigen CD47 (Antigenic surface determinant protein OA3) (Integrin-associated protein) (IAP) (Protein MER6) (CD antigen CD47), CD47, MER6 |
 | IHC image of RACO0401 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Immunofluorescence staining of MCF7 Cells with RACO0401 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in Hela whole cell lysate A549 whole cell lysate HepG2 whole cell lysate All lanes CD47 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 36, 32, 34 kDa. Observed band size: 55 kDa. |
| UniProt Protein Function: | CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3q13.1-q13.2 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response |
| NCBI Summary: | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Code: | Q08722 |
| NCBI GenInfo Identifier: | 1171879 |
| NCBI Gene ID: | 961 |
| NCBI Accession: | Q08722.1 |
| UniProt Related Accession: | Q08722 |
| Molecular Weight: | |
| NCBI Full Name: | Leukocyte surface antigen CD47 |
| NCBI Synonym Full Names: | CD47 molecule |
| NCBI Official Symbol: | CD47 |
| NCBI Official Synonym Symbols: | IAP; OA3; MER6 |
| NCBI Protein Information: | leukocyte surface antigen CD47 |
| UniProt Protein Name: | Leukocyte surface antigen CD47 |
| UniProt Synonym Protein Names: | Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47 |
| Protein Family: | IgE-binding protein |
| UniProt Gene Name: | CD47 |
| UniProt Entry Name: | CD47_HUMAN |