Anti-CD18 Antibody (CAB2173)
- SKU:
- CAB2173
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| 抗体名: | Anti-CD18 Antibody |
| 抗体コード: | CAB2173 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 70-170 of human CD18 (NP_000202.3). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-937, Mouse thymus, Mouse lung, Rat thymus |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 70-170 of human CD18 (NP_000202.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MRGC AADD IMDP TSLA ETQE DHNG GQKQ LSPQ KVTL YLRP GQAA AFNV TFRR AKGY PIDL YYLM DLSY SMLD DLRN VKKL GGDL LRAL NEIT ESGR IGFG S |
| 遺伝子ID: | 3689 |
| Uniprot: | P05107 |
| セルラーロケーション: | Membrane, Single-pass type I membrane protein |
| 計算された分子量: | 84kDa |
| 観察された分子量: | 100kDa |
| 同義語: | CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, ITGB2 |
| バックグラウンド: | This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1). |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc.; Cell surface; Cell adhesion; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: cell surface; membrane; plasma membrane; integrin complex; receptor complex; vesicle; lipid raft Molecular Function:protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; cell adhesion molecule binding; ICAM-3 receptor activity; glycoprotein binding; protein kinase binding Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; apoptosis; cell-matrix adhesion; natural killer cell activation; receptor clustering; activation of NF-kappaB transcription factor; regulation of cell shape; leukocyte adhesion; cellular extravasation; cell-cell signaling; inflammatory response; cell adhesion; toll-like receptor 4 signaling pathway; regulation of peptidyl-tyrosine phosphorylation; aging; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; activated T cell proliferation; leukocyte migration during inflammatory response; heterotypic cell-cell adhesion; positive regulation of angiogenesis; toll-like receptor signaling pathway; innate immune response; endothelial cell migration; blood coagulation; leukocyte migration Disease: Leukocyte Adhesion Deficiency, Type I |
| NCBI Summary: | This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| UniProt Code: | P05107 |
| NCBI GenInfo Identifier: | 124056465 |
| NCBI Gene ID: | 3689 |
| NCBI Accession: | P05107.2 |
| UniProt Secondary Accession: | P05107,Q16418, Q53HS5, Q9UD72, B3KTS8, D3DSM1, |
| UniProt Related Accession: | P05107 |
| Molecular Weight: | 769 |
| NCBI Full Name: | Integrin beta-2 |
| NCBI Synonym Full Names: | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| NCBI Official Symbol: | ITGB2 |
| NCBI Official Synonym Symbols: | LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1 |
| NCBI Protein Information: | integrin beta-2; integrin beta chain, beta 2; complement receptor C3 beta-subunit; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor) |
| UniProt Protein Name: | Integrin beta-2 |
| UniProt Synonym Protein Names: | Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD_antigen: CD18 |
| Protein Family: | Integrin |
| UniProt Gene Name: | ITGB2 |
| UniProt Entry Name: | ITB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD18 antibody (CAB2173) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded Rat lung using CD18 antibody (CAB2173) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat spleen using CD18 antibody (CAB2173) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse spleen using CD18 antibody (CAB2173) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of RAW264.7 cells using CD18 antibody (CAB2173) at dilution of 1:100. Blue: DAPI for nuclear staining. |