Anti-CD11B Antibody (CAB1581)
- SKU:
- CAB1581
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CD11B Antibody |
| 抗体コード: | CAB1581 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD11B (NP_001139280.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse spleen, Mouse lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD11B (NP_001139280.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DTEN AMTF QENA RGFG QSVV QLQG SRVV VGAP QEIV AANQ RGSL YQCD YSTG SCEP IRLQ VPVE AVNM SLGL SLAA TTSP PQLL ACGP TVHQ TCSE NTYV KGLC FLFG SNLR QQPQ KFPE ALRG CPQE DSDI AFLI DGSG SIIP HDFR RMKE FVST VMEQ LKKS KTLF SLMQ YSEE FRIH FTFK EFQN NPNP RSLV KPIT QLLG RTHT ATGI RKVV RELF NITN GARK NAFK ILVV ITDG EKFG DPLG YEDV IPEA DREG VIRY VIGV GDAF RSEK SRQE LNTI ASKP PRDH VFQV NNFE ALKT IQNQ LRE |
| 遺伝子ID: | 3684 |
| Uniprot: | P11215 |
| セルラーロケーション: | Membrane, Single-pass type I membrane protein |
| 計算された分子量: | 127kDa |
| 観察された分子量: | 180kDa |
| 同義語: | CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6, ITGAM, CD11b/ITAM |
| バックグラウンド: | This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes. |
| UniProt Protein Details: | Protein type:Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: cell surface; plasma membrane; integrin complex; nucleus; external side of plasma membrane Molecular Function:heparan sulfate proteoglycan binding; heparin binding; protein binding; opsonin binding; protein heterodimerization activity; metal ion binding; glycoprotein binding Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; activated T cell proliferation; leukocyte migration during inflammatory response; cellular extravasation; toll-like receptor signaling pathway; innate immune response; cell adhesion; blood coagulation; leukocyte migration; toll-like receptor 4 signaling pathway Disease: Systemic Lupus Erythematosus, Susceptibility To, 6 |
| NCBI Summary: | This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| UniProt Code: | P11215 |
| NCBI GenInfo Identifier: | 1708572 |
| NCBI Gene ID: | 3684 |
| NCBI Accession: | P11215.2 |
| UniProt Secondary Accession: | P11215,Q4VAK0, Q4VAK1, Q4VAK2, |
| UniProt Related Accession: | P11215 |
| Molecular Weight: | 1152 |
| NCBI Full Name: | Integrin alpha-M |
| NCBI Synonym Full Names: | integrin, alpha M (complement component 3 receptor 3 subunit) |
| NCBI Official Symbol: | ITGAM |
| NCBI Official Synonym Symbols: | CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6 |
| NCBI Protein Information: | integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit |
| UniProt Protein Name: | Integrin alpha-M |
| UniProt Synonym Protein Names: | CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen: CD11b |
| Protein Family: | Integrin |
| UniProt Gene Name: | ITGAM |
| UniProt Entry Name: | ITAM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD11B antibody (CAB1581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of rat bone marrow using CD11B antibody (CAB1581) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of RAW264.7 cells using CD11B antibody (CAB1581) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of THP-1 cells using CD11B antibody (CAB1581) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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