Anti-CCT3 Antibody (CAB6547)
- SKU:
- CAB6547
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CCT3 Antibody |
| 抗体コード: | CAB6547 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCT3 (NP_005989.3). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, 293T, HepG2, Mouse brain, Mouse heart, Mouse kidney, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCT3 (NP_005989.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MMGH RPVL VLSQ NTKR ESGR KVQS GNIN AAKT IADI IRTC LGPK SMMK MLLD PMGG IVMT NDGN AILR EIQV QHPA AKSM IEIS RTQD EEVG DGTT SVII LAGE MLSV AEHF LEQQ MHPT VVIS AYRK ALDD MIST LKKI SIPV DISD SDMM LNII NSSI TTKA ISRW SSLA CNIA LDAV KMVQ FEEN GRKE IDIK KYAR VEKI PGGI IEDS CVLR GVMI NKDV THPR MRRY IKNP RIVL LDSS LEYK KGES QTDI EITR EEDF TRIL QMEE EYIQ QLCE DIIQ LKPD VVIT EKGI SDLA |
| 遺伝子ID: | 7203 |
| Uniprot: | P49368 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 56kDa/60kDa |
| 観察された分子量: | 60kDa |
| 同義語: | CCT3, CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5 |
| バックグラウンド: | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. |
| UniProt Protein Function: | CCT-gamma: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Chaperone Chromosomal Location of Human Ortholog: 1q23 Cellular Component: chaperonin-containing T-complex; microtubule; cytoskeleton; cytoplasm; plasma membrane; cytosol Molecular Function:protein binding; unfolded protein binding; ATP binding Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; pore complex biogenesis |
| NCBI Summary: | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq, Aug 2010] |
| UniProt Code: | P49368 |
| NCBI GenInfo Identifier: | 66774185 |
| NCBI Gene ID: | 7203 |
| NCBI Accession: | P49368.4 |
| UniProt Secondary Accession: | P49368,Q5SZY1, Q9BR64, A6NE14, |
| UniProt Related Accession: | P49368 |
| Molecular Weight: | 545 |
| NCBI Full Name: | T-complex protein 1 subunit gamma |
| NCBI Synonym Full Names: | chaperonin containing TCP1, subunit 3 (gamma) |
| NCBI Official Symbol: | CCT3 |
| NCBI Official Synonym Symbols: | CCTG; PIG48; TRIC5; CCT-gamma; TCP-1-gamma |
| NCBI Protein Information: | T-complex protein 1 subunit gamma; hTRiC5; T-complex protein 1, gamma subunit; TCP1 (t-complex-1) ring complex, polypeptide 5 |
| UniProt Protein Name: | T-complex protein 1 subunit gamma |
| UniProt Synonym Protein Names: | CCT-gamma; hTRiC5 |
| Protein Family: | Thermosome |
| UniProt Gene Name: | CCT3 |
| UniProt Entry Name: | TCPG_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CCT3 antibody (CAB6547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded mouse heart using CCT3 antibody (CAB6547) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using CCT3 antibody (CAB6547) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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