Anti-CCM2 Antibody (CAB6544)
- SKU:
- CAB6544
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CCM2 Antibody |
| 抗体コード: | CAB6544 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCM2 (NP_113631.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCM2 (NP_113631.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEEE GKKG KKPG IVSP FKRV FLKG EKSR DKKA HEKV TERR PLHT VVLS LPER VEPD RLLS DYIE KEVK YLGQ LTSI PGYL NPSS RTEI LHFI DNAK RAHQ LPGH LTQE HDAV LSLS AYNV KLAW RDGE DIIL RVPI HDIA AVSY VRDD AAHL VVLK TAQD PGIS PSQS LCAE SSRG LSAG SLSE SAVG PVEA CCLV ILAA ESKV AAEE LCCL LGQV FQVV YTES TIDF LDRA IFDG ASTP THHL SLHS DDSS TKVD IKET YEVE ASTF CFPE SVDV GGAS PHSK TISE SELS ASAT ELLQ |
| 遺伝子ID: | 83605 |
| Uniprot: | Q9BSQ5 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 39kDa/42kDa/48kDa/51kDa |
| 観察された分子量: | 55kDa |
| 同義語: | CCM2, C7orf22, OSM, PP10187 |
| バックグラウンド: | This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CCM2: Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions. May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3- dependent p38 activation induced by hyperosmotic shock. Defects in CCM2 are the cause of cerebral cavernous malformations type 2 (CCM2). Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and are usually present clinically during the 3rd to 5th decades of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 7p13 Cellular Component: protein complex; cytoplasm Molecular Function:protein binding Biological Process: integrin-mediated signaling pathway; intercellular junction assembly and maintenance; pericardium development; venous blood vessel morphogenesis; in utero embryonic development; multicellular organism growth; stress-activated MAPK cascade; vasculogenesis; inner ear development; endothelial cell development Disease: Cerebral Cavernous Malformations 2 |
| NCBI Summary: | This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009] |
| UniProt Code: | Q9BSQ5 |
| NCBI GenInfo Identifier: | 74733042 |
| NCBI Gene ID: | 83605 |
| NCBI Accession: | Q9BSQ5.1 |
| UniProt Secondary Accession: | Q9BSQ5,Q71RE5, Q8TAT4, A4D2L4, B3KUV0, D3DVL4, E9PDJ3 F5H0E1, F5H551, |
| UniProt Related Accession: | Q9BSQ5 |
| Molecular Weight: | 444 |
| NCBI Full Name: | Malcavernin |
| NCBI Synonym Full Names: | cerebral cavernous malformation 2 |
| NCBI Official Symbol: | CCM2 |
| NCBI Official Synonym Symbols: | OSM; C7orf22; PP10187 |
| NCBI Protein Information: | malcavernin; cerebral cavernous malformations 2 protein |
| UniProt Protein Name: | Malcavernin |
| UniProt Synonym Protein Names: | Cerebral cavernous malformations 2 protein |
| Protein Family: | Cerebral cavernous malformations protein |
| UniProt Gene Name: | CCM2 |
| UniProt Entry Name: | CCM2_HUMAN |
 | Western blot analysis of extracts of MCF7 cells, using CCM2 antibody (CAB6544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver using CCM2 antibody (CAB6544) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using CCM2 antibody (CAB6544) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using CCM2 antibody (CAB6544) at dilution of 1:100 (40x lens). |
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