Anti-CBX1 Antibody (CAB2247)
- SKU:
- CAB2247
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CBX1 Antibody |
| 抗体コード: | CAB2247 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human CBX1 (NP_006798.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
| 反応性: | Human |
| ポジティブサンプル: | OVCAR3 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human CBX1 (NP_006798.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGKK QNKK KVEE VLEE EEEE YVVE KVLD RRVV KGKV EYLL KWKG FSDE DNTW EPEE NLDC PDLI AEFL QSQK TAHE TDKS EGGK RKAD SDSE DKGE ESKP KKKK EESE KPRG FARG LEPE RIIG ATDS SGEL MFLM KWKN SDEA DLVP AKEA NVKC PQVV ISFY EERL TWHS YPSE DDDK KDDK N |
| 遺伝子ID: | 10951 |
| Uniprot: | P83916 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 21kDa |
| 観察された分子量: | 21kDa |
| 同義語: | CBX1, CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, M31, MOD1, p25beta |
| バックグラウンド: | This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| UniProt Protein Function: | CBX1: Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. Homodimer. Interacts directly with CHAF1A, EMSY, LBR, TIF1/TIF1A and TRIM28/TIF1B PXVXL motif via the chromoshadow domain. Interacts directly with histone H3 methylated at 'Lys-9' via the chromo domain. Interacts with SUV39H1 and SETDB1, SUV420H1 and SUV420H2. Interacts with PRDM6. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1. Expressed in all adult and embryonic tissues. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: nucleoplasm; centric heterochromatin; male pronucleus; female pronucleus; spindle; nuclear heterochromatin; chromatin; chromosome, pericentric region; chromocenter Molecular Function:protein binding; protein homodimerization activity; enzyme binding; chromatin binding Biological Process: negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P83916 |
| NCBI GenInfo Identifier: | 48428808 |
| NCBI Gene ID: | 5077 |
| NCBI Accession: | P83916.1 |
| UniProt Related Accession: | P23760 |
| Molecular Weight: | |
| NCBI Full Name: | Chromobox protein homolog 1 |
| NCBI Synonym Full Names: | paired box 3 |
| NCBI Official Symbol: | PAX3 |
| NCBI Official Synonym Symbols: | WS1; WS3; CDHS; HUP2 |
| NCBI Protein Information: | paired box protein Pax-3 |
| UniProt Protein Name: | Chromobox protein homolog 1 |
| UniProt Synonym Protein Names: | HP1Hsbeta; Heterochromatin protein 1 homolog beta; HP1 beta; Heterochromatin protein p25; M31; Modifier 1 protein; p25beta |
| Protein Family: | Chromobox protein |
| UniProt Gene Name: | CBX1 |
| UniProt Entry Name: | CBX1_HUMAN |
 | Western blot analysis of extracts of OVCAR-3 cells, using CBX1 antibody (CAB2247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human stomach using CBX1 antibody (CAB2247) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using CBX1 antibody (CAB2247) at dilution of 1:100 (40x lens). |
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