Anti-CASP8AP2 Antibody (CAB15770)
- SKU:
- CAB15770
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| 抗体名: | Anti-CASP8AP2 Antibody |
| 抗体コード: | CAB15770 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1733-1982 of human CASP8AP2 (NP_036247.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | U-87MG, K-562 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1733-1982 of human CASP8AP2 (NP_036247.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | TVGA FIDL TQDA SSEA KSEG NHPA LAVE DLGC GVIQ VDED NCKE EKAQ VANR PLKC IVEE TYID LTTE SPSS CEVK KDEL KSEP GSNC DNSE LPGT LHNS HKKR RNIS DLNH PHKK QRKE TDLT NKEK TKKP TQDS CENT EAHQ KKAS KKKA PPVT KDPS SLKA TPGI KDSS AALA TSTS LSAK NVIK KKGE IIIL WTRN DDRE ILLE CQKR GPSF KTFA YLAA KLDK NPNQ VSER FQQL MKLF EKSK CR |
| 遺伝子ID: | 9994 |
| Uniprot: | Q9UKL3 |
| セルラーロケーション: | Cytoplasm, Mitochondrion, Nucleus, PML body |
| 計算された分子量: | 222kDa |
| 観察された分子量: | 215kDa |
| 同義語: | CASP8AP2, CED-4, FLASH, RIP25 |
| バックグラウンド: | This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein. |
| UniProt Protein Function: | CASP8AP2: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF- kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase. |
| UniProt Protein Details: | Protein type:Apoptosis; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 6q15 Cellular Component: PML body; mitochondrion; cytoplasm; nucleus Molecular Function:protein binding; DNA binding; SUMO polymer binding; death receptor binding; apoptotic protease activator activity; caspase activator activity; transcription corepressor activity Biological Process: caspase activation; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; transcription, DNA-dependent; signal transduction; cell cycle |
| NCBI Summary: | This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Nov 2008] |
| UniProt Code: | Q9UKL3 |
| NCBI GenInfo Identifier: | 5533376 |
| NCBI Gene ID: | 9994 |
| NCBI Accession: | AF165161 |
| UniProt Secondary Accession: | Q9UKL3,Q6PH76, Q7LCQ7, Q86YD9, Q9NUQ4, Q9NZV9, Q9P2N1 Q9Y563, A2RUB7, E1P553, |
| UniProt Related Accession: | Q9UKL3 |
| Molecular Weight: | 222,658 Da |
| NCBI Full Name: | Homo sapiens FLASH mRNA, partial cds |
| NCBI Synonym Full Names: | caspase 8 associated protein 2 |
| NCBI Official Symbol: | CASP8AP2 |
| NCBI Official Synonym Symbols: | CED-4; FLASH; RIP25 |
| NCBI Protein Information: | CASP8-associated protein 2 |
| UniProt Protein Name: | CASP8-associated protein 2 |
| UniProt Synonym Protein Names: | FLICE-associated huge protein |
| UniProt Gene Name: | CASP8AP2 |
| UniProt Entry Name: | C8AP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CASP8AP2 antibody (CAB15770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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