Anti-CARD10 Antibody (CAB7368)
- SKU:
- CAB7368
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CARD10 Antibody |
| 抗体コード: | CAB7368 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 863-1032 of human CARD10 (NP_055365.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | SW620, HT-29, Jurkat, 293T, Mouse kidney, Mouse heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 863-1032 of human CARD10 (NP_055365.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LLDL PSSR LDFQ VCPA ESLS GEEL CPSS APGA PKAQ PATP GLGS RIRA IQES VGKK HCLL ELGA RGVR ELVQ NEIY PIVI HVEV TEKN VREV RGLL GRPG WRDS ELLR QCRG SEQV LWGL PCSW VQVP AHEW GHAE ELAK VVRG RILQ EQAR LVWV ECGS SRGC PSSS EA |
| 遺伝子ID: | 29775 |
| Uniprot: | Q9BWT7 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 82kDa/115kDa |
| 観察された分子量: | 80kDa |
| 同義語: | CARD10, BIMP1, CARMA3 |
| バックグラウンド: |
| UniProt Protein Function: | CARD10: a protein containing a caspase recruitment domain (CARD) that functions as an activator of BCL10 and NF-kappaB signaling. CARD is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. CARD proteins function as scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. The CARD domain of CARD14 associates specifically with the CARD domains of CARD10 and BCL10, a signaling protein that activates NF-kB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD14 activates NF-kappaB and induces the phosphorylation of BCL10. A subgroup of primary lymphomas of the central nervous system have higher levels of CARD14 expression. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 22q13.1 Molecular Function:protein binding Biological Process: activation of NF-kappaB-inducing kinase |
| NCBI Summary: | The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9BWT7 |
| NCBI GenInfo Identifier: | 116241281 |
| NCBI Gene ID: | 29775 |
| NCBI Accession: | Q9BWT7.2 |
| UniProt Secondary Accession: | Q9BWT7,Q14CQ8, Q5TFG6, Q8NC81, Q9UGR5, Q9UGR6, Q9Y3H0 |
| UniProt Related Accession: | Q9BWT7 |
| Molecular Weight: | 82,541 Da |
| NCBI Full Name: | Caspase recruitment domain-containing protein 10 |
| NCBI Synonym Full Names: | caspase recruitment domain family member 10 |
| NCBI Official Symbol: | CARD10 |
| NCBI Official Synonym Symbols: | BIMP1; CARMA3 |
| NCBI Protein Information: | caspase recruitment domain-containing protein 10 |
| UniProt Protein Name: | Caspase recruitment domain-containing protein 10 |
| UniProt Synonym Protein Names: | CARD-containing MAGUK protein 3; Carma 3 |
| Protein Family: | Caspase recruitment domain-containing protein |
| UniProt Gene Name: | CARD10 |
| UniProt Entry Name: | CAR10_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CARD10 antibody (CAB7368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using CARD10 antibody (CAB7368) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using CARD10 antibody (CAB7368) at dilution of 1:100 (40x lens). |
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