Anti-CAPN2 Antibody (CAB1861)[KO Validated]
![Anti-CAPN2 Antibody (CAB1861)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/26107/25955/anti-capn2-antibody-cab1861ko-validated__68659__98021.1645806411.jpg?c=1 "Anti-CAPN2 Antibody (CAB1861)[KO Validated]")
- SKU:
- CAB1861
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
![Anti-CAPN2 Antibody (CAB1861)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/26107/25955/anti-capn2-antibody-cab1861ko-validated__68659__98021.1645806411.jpg?c=1&imbypass=on "Anti-CAPN2 Antibody (CAB1861)[KO Validated]")
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Description
| 抗体名: | Anti-CAPN2 Antibody [KO Validated] |
| 抗体コード: | CAB1861 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human CAPN2 (NP_001739.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | H460, A-549, HeLa, Mouse kidney, Mouse lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human CAPN2 (NP_001739.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | SDTY KKWK LTKM DGNW RRGS TAGG CRNY PNTF WMNP QYLI KLEE EDED EEDG ESGC TFLV GLIQ KHRR RQRK MGED MHTI GFGI YEVP EELS GQTN IHLS KNFF LTNR ARER SDTF INLR EVLN RFKL PPGE YILV PSTF EPNK DGDF CIRV FSEK KADY QAVD DEIE ANLE EFDI SEDD IDDG FRRL FAQL AGED AEIS AFEL QTIL RRVL AKRQ DIKS DGFS IETC KIMV DMLD SDGS GKLG LKEF YILW TKIQ KYQK IYRE IDVD RSGT MNSY EMRK ALEE AGFK MPCQ LHQV IVAR FADD QLII DFDN FVRC LVRL ETLF KIFK QLD |
| 遺伝子ID: | 824 |
| Uniprot: | P17655 |
| セルラーロケーション: | Cell membrane, Cytoplasm |
| 計算された分子量: | 71kDa/79kDa |
| 観察された分子量: | 80kDa |
| 同義語: | CAPN2, CANP2, CANPL2, CANPml, mCANP, calpain 2 |
| バックグラウンド: | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs. |
| NCBI Summary: | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| UniProt Code: | P17655 |
| NCBI GenInfo Identifier: | 317373596 |
| NCBI Gene ID: | 824 |
| NCBI Accession: | P17655.6 |
| UniProt Secondary Accession: | P17655,Q16738, Q6PJT3, Q8WU26, Q9HBB1, A6NDG7, B7ZA96 E7ES58, |
| UniProt Related Accession: | P17655 |
| Molecular Weight: | 71,476 Da |
| NCBI Full Name: | Calpain-2 catalytic subunit |
| NCBI Synonym Full Names: | calpain 2 |
| NCBI Official Symbol: | CAPN2 |
| NCBI Official Synonym Symbols: | CANP2; mCANP; CANPL2; CANPml |
| NCBI Protein Information: | calpain-2 catalytic subunit |
| UniProt Protein Name: | Calpain-2 catalytic subunit |
| UniProt Synonym Protein Names: | Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain |
| Protein Family: | Calpain |
| UniProt Gene Name: | CAPN2 |
![Anti-CAPN2 Antibody [KO Validated] (CAB1861)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/m/271/A1861_1__23374.jpg) | Western blot analysis of extracts of various cell lines, using CAPN2 antibody (CAB1861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
![Anti-CAPN2 Antibody [KO Validated] (CAB1861)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/w/294/A1861_2__19532.jpg) | Western blot analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using CAPN2 antibody (CAB1861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![Anti-CAPN2 Antibody [KO Validated] (CAB1861)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/a/928/A1861_3__40014.jpg) | Immunofluorescence analysis of U2OS cells using CAPN2 antibody (CAB1861) at dilution of 1:100. Blue: DAPI for nuclear staining. |