Anti-CA4 Antibody (CAB2677)
- SKU:
- CAB2677
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CA4 Antibody |
| 抗体コード: | CAB2677 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-270 of human CA4 (NP_000708.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | SH-SY5Y, MCF7, Mouse lung, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-270 of human CA4 (NP_000708.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AESH WCYE VQAE SSNY PCLV PVKW GGNC QKDR QSPI NIVT TKAK VDKK LGRF FFSG YDKK QTWT VQNN GHSV MMLL ENKA SISG GGLP APYQ AKQL HLHW SDLP YKGS EHSL DGEH FAME MHIV HEKE KGTS RNVK EAQD PEDE IAVL AFLV EAGT QVNE GFQP LVEA LSNI PKPE MSTT MAES SLLD LLPK EEKL RHYF RYLG SLTT PTCD EKVV WTVF REPI QLHR EQIL AFSQ KLYY DKEQ TVSM KDNV |
| 遺伝子ID: | 762 |
| Uniprot: | P22748 |
| セルラーロケーション: | Cell membrane, GPI-anchor, Lipid-anchor |
| 計算された分子量: | 11kDa/35kDa |
| 観察された分子量: | 35kDa |
| 同義語: | CA4, CAIV, Car4, RP17 |
| バックグラウンド: | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. |
| UniProt Protein Function: | CA4: Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. Defects in CA4 are the cause of retinitis pigmentosa type 17 (RP17). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP17 inheritance is autosomal dominant. Defective acid overload removal from retina and retinal epithelium, due to mutant CA4, is responsible for photoreceptor degeneration, indicating that impaired pH homeostasis is the most likely cause underlying the RP17 phenotype. Belongs to the alpha-carbonic anhydrase family. |
| UniProt Protein Details: | Protein type:Lyase; Energy Metabolism - nitrogen; Membrane protein, GPI anchor; EC 4.2.1.1 Chromosomal Location of Human Ortholog: 17q23 Cellular Component: Golgi apparatus; cell surface; sarcoplasmic reticulum; rough endoplasmic reticulum; basolateral plasma membrane; brush border membrane; secretory granule membrane; ER-Golgi intermediate compartment; trans-Golgi network; anchored to external side of plasma membrane; membrane; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; sarcolemma Molecular Function:carbonate dehydratase activity; protein binding; zinc ion binding Biological Process: response to drug; response to steroid hormone stimulus; bicarbonate transport; organ development; one-carbon compound metabolic process Disease: Retinitis Pigmentosa 17 |
| NCBI Summary: | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P22748 |
| NCBI GenInfo Identifier: | 115465 |
| NCBI Gene ID: | 762 |
| NCBI Accession: | P22748.2 |
| UniProt Secondary Accession: | P22748,Q64444, P48284, |
| UniProt Related Accession: | P22748 |
| Molecular Weight: | 312 |
| NCBI Full Name: | Carbonic anhydrase 4 |
| NCBI Synonym Full Names: | carbonic anhydrase IV |
| NCBI Official Symbol: | CA4 |
| NCBI Official Synonym Symbols: | CAIV; Car4; RP17 |
| NCBI Protein Information: | carbonic anhydrase 4; CA-IV; carbonic dehydratase IV; carbonate dehydratase IV |
| UniProt Protein Name: | Carbonic anhydrase 4 |
| UniProt Synonym Protein Names: | Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV |
| Protein Family: | Carbonic anhydrase |
| UniProt Gene Name: | CA4 |
| UniProt Entry Name: | CAH4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CA4 antibody (CAB2677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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