Anti-Bub3 Antibody (CAB8831)
- SKU:
- CAB8831
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-Bub3 Antibody |
| 抗体コード: | CAB8831 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthesized peptide derived from human Bub3 |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, 293T, MCF7, C2C12, Mouse liver, Rat lung |
| 免疫原: | A synthesized peptide derived from human Bub3 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 9184 |
| Uniprot: | O43684 |
| セルラーロケーション: | |
| 計算された分子量: | 40kDa |
| 観察された分子量: | 40KDa |
| 同義語: | BUB3L, hBUB3 |
| バックグラウンド: | This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Protein Function: | BUB3: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Belongs to the WD repeat BUB3 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 10q26 Cellular Component: kinetochore; nucleoplasm; cytosol Molecular Function:protein binding Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; meiotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; attachment of spindle microtubules to kinetochore |
| NCBI Summary: | This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43684 |
| NCBI GenInfo Identifier: | 7387554 |
| NCBI Gene ID: | 9184 |
| NCBI Accession: | O43684.1 |
| UniProt Secondary Accession: | O43684,O43685, A6NJ42, B2R6E7, D3DRE9, |
| UniProt Related Accession: | O43684 |
| Molecular Weight: | 328 |
| NCBI Full Name: | Mitotic checkpoint protein BUB3 |
| NCBI Synonym Full Names: | BUB3 mitotic checkpoint protein |
| NCBI Official Symbol: | BUB3 |
| NCBI Official Synonym Symbols: | BUB3L; hBUB3 |
| NCBI Protein Information: | mitotic checkpoint protein BUB3; mitotic checkpoint component; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog |
| UniProt Protein Name: | Mitotic checkpoint protein BUB3 |
| Protein Family: | BUB3-interacting and GLEBS motif-containing protein |
| UniProt Gene Name: | BUB3 |
| UniProt Entry Name: | BUB3_HUMAN |
 | Western blot - Bub3 Rabbit mAb (CAB8831) |
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