Anti-BRCC3 Antibody (CAB7995)

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SKU:
CAB7995
Product type:
Antibody
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
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Description

system_update_altデータシートsystem_update_alt安全性データシート
抗体名:BRCC3 Rabbit Polyclonal Antibody
抗体コード:CAB7995
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human BRCC3 (NP_077308.1).
申し込み:WB
推奨希釈:WB 1:500 - 1:2000
反応性:Human, Mouse, Rat
ポジティブサンプル:HeLa, MCF7, Jurkat, 293T, Mouse liver, Mouse kidney, Mouse brain, Rat liver
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human BRCC3 (NP_077308.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MAVQ VVQA VQAV HLES DAFL VCLN HALS TEKE EVMG LCIG ELND DTRS DSKF AYTG TEMR TVAE KVDA VRIV HIHS VIIL RRSD KRKD RVEI SPEQ LSAA STEA ERLA ELTG RPMR VVGW YHSH PHIT VWPS HVDV RTQA MYQM MDQG FVGL IFSC FIED KNTK TGRV LYTC FQSI QAQK SSES LHGP RDFW SSSQ HISI EGQK EEER YERI EIPI HIVP HVTI GKVC LESA VELP KILC QEEQ DAYR RIHS LTHL DSVT KIHN GSVF TKNL CSQM SAVS GPLL QWLE DRLE QNQQ HLQE LQQE KEEL MQEL SSLE
遺伝子ID:79184
Uniprot:P46736
セルラーロケーション:Nucleus
計算された分子量:23kDa/28kDa/33kDa/36kDa
観察された分子量:33kDa/36kDa
同義語:BRCC3, BRCC36, C6.1A, CXorf53
バックグラウンド:This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.
UniProt Protein Function:BRCC3: Metalloprotease that specifically cleaves 'Lys-63'- linked polyubiquitin chains. Does not have activity toward 'Lys- 48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'- specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA. Belongs to the peptidase M67A family. BRCC36 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Oncoprotein; EC 3.4.19.-; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; nuclear ubiquitin ligase complex; nucleus; ubiquitin ligase complex

Molecular Function:protein binding; metallopeptidase activity; metal ion binding; ubiquitin-specific protease activity; polyubiquitin binding; enzyme regulator activity

Biological Process: positive regulation of DNA repair; regulation of catalytic activity; double-strand break repair; response to ionizing radiation; G2/M transition DNA damage checkpoint; proteolysis; response to X-ray

NCBI Summary:This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]
UniProt Code:P46736
NCBI GenInfo Identifier:20532383
NCBI Gene ID:79184
NCBI Accession:P46736.2
UniProt Secondary Accession:P46736,Q16107, Q53YX5, Q9BTZ6, A6QRF8, A6QRF9, A8MUX5 A8MWH0, A9Z1Y0, A9Z1Y5, B1B062, B4DQN7,
UniProt Related Accession:P46736
Molecular Weight:316
NCBI Full Name:Lys-63-specific deubiquitinase BRCC36
NCBI Synonym Full Names:BRCA1/BRCA2-containing complex, subunit 3
NCBI Official Symbol:BRCC3  
NCBI Official Synonym Symbols:C6.1A; BRCC36; CXorf53  
NCBI Protein Information:lys-63-specific deubiquitinase BRCC36; BRISC complex subunit BRCC36; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 36
UniProt Protein Name:Lys-63-specific deubiquitinase BRCC36
UniProt Synonym Protein Names:BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36
Protein Family:Lys-63-specific deubiquitinase
UniProt Gene Name:BRCC3  
UniProt Entry Name:BRCC3_HUMAN
Anti-BRCC3 Polyclonal Antibody (CAB7995)Western blot analysis of extracts of various cell lines, using BRCC3 antibody (CAB7995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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