Anti-BLZF1 Antibody (CAB6430)
- SKU:
- CAB6430
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-BLZF1 Antibody |
| 抗体コード: | CAB6430 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human BLZF1 (NP_003657.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, NIH/3T3, U-251MG, Jurkat, Mouse skeletal muscle |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human BLZF1 (NP_003657.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MTTK NLET KVTV TSSP IRGA GDGM ETEE PPKS VEVT SGVQ SRKH HSLQ SPWK KAVP SESP GVLQ LGKM LTEK AMEV KAVR ILVP KAAI THDI PNKN TKVK SLGH HKGE FLGQ SEGV IEPN KELS EVKN VLEK LKNS ERRL LQDK EGLS NQLR VQTE VNRE LKKL LVAS VGDD LQYH FERL AREK NQLI LENE ALGR NTAQ LSEQ LERM SIQC DVWR SKFL ASRV MADE LTNS RAAL QRQN RDAH GAIQ DLLS EREQ FRQE MIAT QKLL EELL VSLQ WGRE QTYS PSVQ PHST AELA LTNH KLAK AVNS HLLG NVGI NNQK KIPS TVEF CSTP AEKM AETV LRIL DPVT CKES SPDN PFFE SSPT TLLA TKKN IGRF HPYT RYEN ITFN CCNH CRGE LIAL |
| 遺伝子ID: | 8548 |
| Uniprot: | Q9H2G9 |
| セルラーロケーション: | Cytoplasm, Golgi apparatus lumen, Nucleus |
| 計算された分子量: | 19kDa/44kDa |
| 観察された分子量: | 45kDa |
| 同義語: | BLZF1, GOLGIN-45, JEM-1, JEM-1s, JEM1, golgin-45 |
| バックグラウンド: |
| UniProt Protein Function: | BLZF1: Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1q24 Cellular Component: Golgi membrane; nucleoplasm; nucleus Molecular Function:enzyme binding; protein binding; ubiquitin protein ligase binding Biological Process: cell proliferation; Golgi organization and biogenesis; Golgi to plasma membrane protein transport; regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | Q9H2G9 |
| NCBI GenInfo Identifier: | 116242501 |
| NCBI Gene ID: | 8548 |
| NCBI Accession: | Q9H2G9.2 |
| UniProt Secondary Accession: | Q9H2G9,O15298, Q5T531, Q5T533, Q9GZX4, |
| UniProt Related Accession: | Q9H2G9 |
| Molecular Weight: | 19,362 Da |
| NCBI Full Name: | Golgin-45 |
| NCBI Synonym Full Names: | basic leucine zipper nuclear factor 1 |
| NCBI Official Symbol: | BLZF1 |
| NCBI Official Synonym Symbols: | JEM1; JEM-1; JEM-1s; GOLGIN-45 |
| NCBI Protein Information: | golgin-45 |
| UniProt Protein Name: | Golgin-45 |
| UniProt Synonym Protein Names: | Basic leucine zipper nuclear factor 1; JEM-1; p45 basic leucine-zipper nuclear factor |
| Protein Family: | Golgin |
| UniProt Gene Name: | BLZF1 |
| UniProt Entry Name: | GO45_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BLZF1 antibody (CAB6430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human stomach using BLZF1 antibody (CAB6430) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using BLZF1 antibody (CAB6430). Blue: DAPI for nuclear staining. |
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