Anti-Beta-Tubulin Mouse Monoclonal Antibody (CABC021)
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- SKU:
- CABC021
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Hamster
- Reactivity:
- Monkey
- Reactivity:
- Goat
- Reactivity:
- Chlamydomonas Reinhardtii
- Host Species:
- Mouse
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Biology
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Description
| 抗体名: | Anti-Beta-Tubulin Mouse Monoclonal Antibody |
| 抗体コード: | CABC021 |
| 抗体サイズ: | 100 uL |
| 申し込み: | WB IHC IF IP |
| 反応性: | Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii |
| 宿主種: | Mouse |
| 免疫原: | Recombinant protein of human Beta-Tubulin |
| 申し込み: | WB IHC IF IP |
| 推奨希釈: | WB 1:5000 - 1:10000 IHC 1:50- 1:200 IF 1:50- 1:200 IP 1:50- 1:200 |
| 反応性: | Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii |
| ポジティブサンプル: | SH-SY5Y, MCF-7, HepG2, HeLa, Mouse brain, Mouse lung, Rat brain |
| 免疫原: | Recombinant protein of human Beta-Tubulin |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 203068 |
| Uniprot: | P07437 |
| セルラーロケーション: | Cytoplasm, cytoskeleton |
| 計算された分子量: | 49kDa |
| 観察された分子量: | 50kDa |
 | Western blot analysis of extracts of various cell lines, using Beta-Tubulin antibody (CABC021) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using beta-Tubulin Mouse mAb (CABC021) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat stomach using beta-Tubulin Mouse mAb (CABC021) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using Beta-Tubulin Mouse mAb (CABC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using Beta-Tubulin Mouse mAb (CABC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
| 同義語: | CDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, TUBB5, Beta Tubulin, TUBB, Beta-Tubulin |
| バックグラウンド: | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. |
| UniProt Protein Function: | TUBB: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO/C1orf88. Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Belongs to the tubulin family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 6p21.33 Cellular Component: cytoskeleton; extracellular region; microtubule; nuclear envelope lumen; nucleus; protein complex Molecular Function:GTP binding; GTPase activating protein binding; GTPase activity; MHC class I protein binding; protein binding; protein complex binding; protein domain specific binding; structural constituent of cytoskeleton; structural molecule activity; ubiquitin protein ligase binding Biological Process: cell division; cell motility; cellular process; cytoskeleton-dependent intracellular transport; G2/M transition of mitotic cell cycle; metabolic process; microtubule-based process; mitotic cell cycle; natural killer cell mediated cytotoxicity; organelle organization and biogenesis; spindle assembly Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 6 |
| NCBI Summary: | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] |
| UniProt Code: | P07437 |
| NCBI GenInfo Identifier: | 56757569 |
| NCBI Gene ID: | 203068 |
| NCBI Accession: | P07437.2 |
| UniProt Secondary Accession: | P07437,P05218, Q8WUC1, Q9CY33, |
| UniProt Related Accession: | P07437 |
| Molecular Weight: | 49,671 Da |
| NCBI Full Name: | Tubulin beta chain |
| NCBI Synonym Full Names: | tubulin beta class I |
| NCBI Official Symbol: | TUBB |
| NCBI Official Synonym Symbols: | M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56 |
| NCBI Protein Information: | tubulin beta chain |
| UniProt Protein Name: | Tubulin beta chain |
| UniProt Synonym Protein Names: | Tubulin beta-5 chain |
| Protein Family: | Tubulin |
| UniProt Gene Name: | TUBB |
| UniProt Entry Name: | TBB5_HUMAN |