Anti-Beta-Tubulin Antibody (CABC015)
- SKU:
- CABC015
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Beta-Tubulin Antibody |
| 抗体コード: | CABC015 |
| 抗体サイズ: | 100 uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human Beta-Tubulin (NP_006077.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SH-SY5Y, HeLa, A-549, MCF7, HepG2, Mouse eye, Rat spinal cord |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human Beta-Tubulin (NP_006077.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | SDLQ LERI SVYY NEAS SHKY VPRA ILVD LEPG TMDS VRSG AFGH LFRP DNFI FGQS GAGN NWAK GHYT EGAE LVDS VLDV VRKE CENC DCLQ GFQL THSL GGGT GSGM GTLL ISKV REEY PDRI MNTF SVVP SPKV SDTV VEPY NATL SIHQ LVEN TDET YCID NEAL YDIC FRTL KLAT PTYG DLNH LVSA TMSG VTTS LRFP GQLN ADLR KLAV NMVP F |
| 遺伝子ID: | 203068 |
| Uniprot: | P07437 |
| セルラーロケーション: | Cytoplasm, cytoskeleton |
| 計算された分子量: | 49kDa |
| 観察された分子量: | 50kDa |
 | Western blot analysis of extracts of various cell lines, using TUBB antibody (CABC015) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry of paraffin-embedded HepG2 cells using beta-Tubulin antibody (CABC015) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded HepG2 cells treated by oleic acid using beta-Tubulin antibody (CABC015) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded HepG2 cells treated by oleic acid using Beta-Tubulin antibody (CABC015) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A431 cells using Beta-Tubulin antibody (CABC015) at dilution of 1:100. Blue: DAPI for nuclear staining. |
| 同義語: | CDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, TUBB5, Beta Tubulin, TUBB, Beta-Tubulin |
| バックグラウンド: | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. |
| UniProt Protein Function: | TUBB: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO/C1orf88. Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Belongs to the tubulin family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 6p21.33 Cellular Component: cytoskeleton; extracellular region; microtubule; nuclear envelope lumen; nucleus; protein complex Molecular Function:GTP binding; GTPase activating protein binding; GTPase activity; MHC class I protein binding; protein binding; protein complex binding; protein domain specific binding; structural constituent of cytoskeleton; structural molecule activity; ubiquitin protein ligase binding Biological Process: cell division; cell motility; cellular process; cytoskeleton-dependent intracellular transport; G2/M transition of mitotic cell cycle; metabolic process; microtubule-based process; mitotic cell cycle; natural killer cell mediated cytotoxicity; organelle organization and biogenesis; spindle assembly Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 6 |
| NCBI Summary: | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] |
| UniProt Code: | P07437 |
| NCBI GenInfo Identifier: | 56757569 |
| NCBI Gene ID: | 203068 |
| NCBI Accession: | P07437.2 |
| UniProt Secondary Accession: | P07437,P05218, Q8WUC1, Q9CY33, |
| UniProt Related Accession: | P07437 |
| Molecular Weight: | 49,671 Da |
| NCBI Full Name: | Tubulin beta chain |
| NCBI Synonym Full Names: | tubulin beta class I |
| NCBI Official Symbol: | TUBB |
| NCBI Official Synonym Symbols: | M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56 |
| NCBI Protein Information: | tubulin beta chain |
| UniProt Protein Name: | Tubulin beta chain |
| UniProt Synonym Protein Names: | Tubulin beta-5 chain |
| Protein Family: | Tubulin |
| UniProt Gene Name: | TUBB |
| UniProt Entry Name: | TBB5_HUMAN |