Anti-BAIAP2 Antibody (CAB5337)
- SKU:
- CAB5337
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-BAIAP2 Antibody |
| 抗体コード: | CAB5337 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 362-521 of human BAIAP2 (NP_059344.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | A-549, K-562, SW620, 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 362-521 of human BAIAP2 (NP_059344.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | PRSS SMAA GLER NGRM RVKA IFSH AAGD NSTL LSFK EGDL ITLL VPEA RDGW HYGE SEKT KMRG WFPF SYTR VLDS DGSD RLHM SLQQ GKSS STGN LLDK DDLA IPPP DYGA ASRA FPAQ TASG FKQR PYSV AVPA FSQG LDDY GARS MSSG SGTL VSTV |
| 遺伝子ID: | 10458 |
| Uniprot: | Q9UQB8 |
| セルラーロケーション: | Cell projection, Cytoplasm, Membrane, Peripheral membrane protein, cytoskeleton, filopodium, ruffle |
| 計算された分子量: | 56kDa/57kDa/59kDa/60kDa |
| 観察された分子量: | 61-65kDa |
| 同義語: | BAIAP2, BAP2, FLAF3, IRSP53 |
| バックグラウンド: | The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | IRSp53: Adapter protein that links membrane-bound small G- proteins to cytoplasmic effector proteins. Necessary for CDC42- mediated reorganization of the actin cytoskeleton and for RAC1- mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Homodimer. Interacts with CDC42 and RAC1 that have been activated by GTP binding. Interacts with ATN1, BAI1, EPS8, SHANK1, SHANK2, SHANK3, WASF1 and WASF2. Interacts with ENAH after recruitment of CDC42. Interacts with TIAM1 and DIAPH1. Interacts (via SH3 domain) with E.coli effector protein EspF(U) (via PXXP motifs). Interacts with E.coli intimin receptor Tir. Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 17q25 Cellular Component: nucleoplasm; ruffle; neuron projection; cytoplasm; plasma membrane; cytosol; actin cytoskeleton; filopodium Molecular Function:protein C-terminus binding; identical protein binding; protein binding; cytoskeletal adaptor activity Biological Process: actin crosslink formation; regulation of cell shape; actin filament bundle formation; plasma membrane organization and biogenesis; response to bacterium; axonogenesis; regulation of actin cytoskeleton organization and biogenesis; insulin receptor signaling pathway; dendrite development; regulation of synaptic plasticity; innate immune response; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009] |
| UniProt Code: | Q9UQB8 |
| NCBI GenInfo Identifier: | 73917636 |
| NCBI Gene ID: | 10458 |
| NCBI Accession: | Q9UQB8.1 |
| UniProt Secondary Accession: | Q9UQB8,O43858, Q53HB1, Q86WC1, Q8N5C0, Q96CR7, Q9UBR3 Q9UQ43, |
| UniProt Related Accession: | Q9UQB8 |
| Molecular Weight: | 521 |
| NCBI Full Name: | Brain-specific angiogenesis inhibitor 1-associated protein 2 |
| NCBI Synonym Full Names: | BAI1-associated protein 2 |
| NCBI Official Symbol: | BAIAP2 |
| NCBI Official Synonym Symbols: | BAP2; FLAF3; IRSP53 |
| NCBI Protein Information: | brain-specific angiogenesis inhibitor 1-associated protein 2; IRS-58; IRSp53/58; fas ligand-associated factor 3; insulin receptor substrate p53/p58; insulin receptor substrate protein of 53 kDa |
| UniProt Protein Name: | Brain-specific angiogenesis inhibitor 1-associated protein 2 |
| UniProt Synonym Protein Names: | Fas ligand-associated factor 3; FLAF3; Insulin receptor substrate p53/p58; IRS-58; IRSp53/58; Insulin receptor substrate protein of 53 kDa; IRSp53; Insulin receptor substrate p53 |
| Protein Family: | Brain-specific angiogenesis inhibitor 1-associated protein |
| UniProt Gene Name: | BAIAP2 |
| UniProt Entry Name: | BAIP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BAIAP2 antibody (CAB5337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat kidney using BAIAP2 antibody (CAB5337) at dilution of 1:200 (40x lens). |
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