Anti-BAF250 Antibody (CAB16648)
- SKU:
- CAB16648
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Neuroscience
Frequently bought together:
Description
| 抗体名: | Anti-BAF250 Antibody |
| 抗体コード: | CAB16648 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of mouse BAF250 (NP_001074288.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, Mouse testis |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of mouse BAF250 (NP_001074288.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | NMGT GAPQ PNLM PSTP DSGM YSPS RYPP QQQQ QQQQ QHDS YGNQ FSTQ GTPS SSPF PSQQ TTMY QQQQ QNYK RPMD GTYG PPAK RHEG EMYS VPYS AGQG Q |
| 遺伝子ID: | 93760 |
| Uniprot: | A2BH40 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 206kDa/218kDa/242kDa |
| 観察された分子量: | 260kDa |
| 同義語: | ARID1A, B120, BAF250, BAF250a, BM029, C1orf4, CSS2, ELD, MRD14, OSA1, P270, SMARCF1, hELD, hOSA1 |
| バックグラウンド: | This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (PubMed:17640523). |
| UniProt Code: | A2BH40 |
| NCBI GenInfo Identifier: | 124249109 |
| NCBI Gene ID: | 93760 |
| NCBI Accession: | NP_001074288.1 |
| UniProt Secondary Accession: | A2BH40,Q640Q1, Q925Q1, |
| UniProt Related Accession: | A2BH40 |
| Molecular Weight: | 206,488 Da |
| NCBI Full Name: | AT-rich interactive domain-containing protein 1A isoform 1 |
| NCBI Synonym Full Names: | AT rich interactive domain 1A (SWI-like) |
| NCBI Official Symbol: | Arid1a |
| NCBI Official Synonym Symbols: | Osa1; BAF250a; Smarcf1; 1110030E03Rik |
| NCBI Protein Information: | AT-rich interactive domain-containing protein 1A |
| UniProt Protein Name: | AT-rich interactive domain-containing protein 1A |
| UniProt Synonym Protein Names: | BRG1-associated factor 250; BAF250; BRG1-associated factor 250a; BAF250A; Osa homolog 1; SWI-like protein; SWI/SNF complex protein p270; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1 |
| UniProt Gene Name: | Arid1a |
 | Western blot analysis of extracts of various cell lines, using BAF250 antibody (CAB16648) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
View AllClose