Anti-B3GAT1 Antibody (CAB9871)
- SKU:
- CAB9871
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-B3GAT1 Antibody |
| 抗体コード: | CAB9871 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-334 of human B3GAT1 (NP_473366.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | SW480, HT-1080, BT-474 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-334 of human B3GAT1 (NP_473366.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VHKD EGSD PRRE TPPG ADPR EYCT SDRD IVEV VRTE YVYT RPPP WSDT LPTI HVVT PTYS RPVQ KAEL TRMA NTLL HVPN LHWL VVED APRR TPLT ARLL RDTG LNYT HLHV ETPR NYKL RGDA RDPR IPRG TMQR NLAL RWLR ETFP RNSS QPGV VYFA DDDN TYSL ELFE EMRS TRRV SVWP VAFV GGLR YEAP RVNG AGKV VGWK TVFD PHRP FAID MAGF AVNL RLIL QRSQ AYFK LRGV KGGY QESS LLRE LVTL NDLE PKAA NCTK ILVW HTRT EKPV LVNE GKKG FTDP SVEI |
| 遺伝子ID: | 27087 |
| Uniprot: | Q9P2W7 |
| セルラーロケーション: | Golgi apparatus membrane, Single-pass type II membrane protein |
| 計算された分子量: | 38kDa/39kDa |
| 観察された分子量: | 38kDa |
| 同義語: | B3GAT1, CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1, beta-1 |
| バックグラウンド: | The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. |
| UniProt Protein Function: | B3GAT1: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. Belongs to the glycosyltransferase 43 family. |
| UniProt Protein Details: | Protein type:EC 2.4.1.135; Glycan Metabolism - chondroitin sulfate biosynthesis; Transferase; Membrane protein, integral; Glycan Metabolism - heparan sulfate biosynthesis Chromosomal Location of Human Ortholog: 11q25 Cellular Component: Golgi membrane Molecular Function:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Biological Process: carbohydrate metabolic process; chondroitin sulfate metabolic process; chondroitin sulfate proteoglycan biosynthetic process; glycosaminoglycan metabolic process |
| NCBI Summary: | The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9P2W7 |
| NCBI GenInfo Identifier: | 205830910 |
| NCBI Gene ID: | 27087 |
| NCBI Accession: | Q9P2W7.2 |
| UniProt Secondary Accession: | Q9P2W7,Q96FS7, |
| UniProt Related Accession: | Q9P2W7 |
| Molecular Weight: | 38,256 Da |
| NCBI Full Name: | Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 |
| NCBI Synonym Full Names: | beta-1,3-glucuronyltransferase 1 |
| NCBI Official Symbol: | B3GAT1 |
| NCBI Official Synonym Symbols: | NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP |
| NCBI Protein Information: | galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 |
| UniProt Protein Name: | Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 |
| UniProt Synonym Protein Names: | Beta-1,3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; GlcUAT-P |
| Protein Family: | Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase |
| UniProt Gene Name: | B3GAT1 |
| UniProt Entry Name: | B3GA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using B3GAT1 antibody (CAB9871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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