Anti-ATP1B1 Antibody (CAB12403)
- SKU:
- CAB12403
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ATP1B1 Antibody |
| 抗体コード: | CAB12403 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 65-240 of human ATP1B1 (NP_001668.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-251MG, Mouse brain, Mouse liver, Mouse kidney, Rat kidney, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 65-240 of human ATP1B1 (NP_001668.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | KPTY QDRV APPG LTQI PQIQ KTEI SFRP NDPK SYEA YVLN IVRF LEKY KDSA QRDD MIFE DCGD VPSE PKER GDFN HERG ERKV CRFK LEWL GNCS GLND ETYG YKEG KPCI IIKL NRVL GFKP KPPK NESL ETYP VMKY NPNV LPVQ CTGK RDED KDKV GNVE YFGL GNSP GFPL |
| 遺伝子ID: | 481 |
| Uniprot: | P05026 |
| セルラーロケーション: | Cell membrane, Single-pass type II membrane protein |
| 計算された分子量: | 34kDa/35kDa |
| 観察された分子量: | 50kDa |
| 同義語: | ATP1B1, ATP1B |
| バックグラウンド: | The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. |
| UniProt Protein Function: | ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q24 Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; intracellular; membrane; myelin sheath; plasma membrane; sarcolemma; sodium:potassium-exchanging ATPase complex Molecular Function:ATP binding; ATPase activator activity; ATPase activity; ATPase binding; drug binding; potassium ion binding; protein binding; protein C-terminus binding; sodium ion binding; sodium:potassium-exchanging ATPase activity Biological Process: ATP metabolic process; blood coagulation; cardiac muscle contraction; cell adhesion; cellular calcium ion homeostasis; cellular potassium ion homeostasis; cellular sodium ion homeostasis; establishment and/or maintenance of transmembrane electrochemical gradient; leukocyte migration; positive regulation of ATPase activity; positive regulation of defense response to virus by host; potassium ion import; protein stabilization; regulation of gene expression; response to hypoxia; transmembrane transport Disease: Hypertension, Essential |
| NCBI Summary: | The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010] |
| UniProt Code: | P05026 |
| NCBI GenInfo Identifier: | 114392 |
| NCBI Gene ID: | 481 |
| NCBI Accession: | P05026.1 |
| UniProt Secondary Accession: | P05026,Q5TGZ3, |
| UniProt Related Accession: | P05026 |
| Molecular Weight: | 34,893 Da |
| NCBI Full Name: | Sodium/potassium-transporting ATPase subunit beta-1 |
| NCBI Synonym Full Names: | ATPase Na+/K+ transporting subunit beta 1 |
| NCBI Official Symbol: | ATP1B1 |
| NCBI Official Synonym Symbols: | ATP1B |
| NCBI Protein Information: | sodium/potassium-transporting ATPase subunit beta-1 |
| UniProt Protein Name: | Sodium/potassium-transporting ATPase subunit beta-1 |
| UniProt Synonym Protein Names: | Sodium/potassium-dependent ATPase subunit beta-1 |
| UniProt Gene Name: | ATP1B1 |
| UniProt Entry Name: | AT1B1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATP1B1 antibody (CAB12403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of C6 cells using ATP1B1 antibody (CAB12403) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using ATP1B1 antibody (CAB12403) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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