Anti-ATP12A Antibody (CAB14372)
- SKU:
- CAB14372
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-ATP12A Antibody |
| 抗体コード: | CAB14372 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 890-980 of human ATP12A (NP_001172014.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | A-431, 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 890-980 of human ATP12A (NP_001172014.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | YFTV YAQE GFLP RTLI NLRV EWEK DYVN DLKD SYGQ EWTR YQRE YLEW TGYT AFFV GILV QQIA DLII RKTR RNSI FQQG LFRN KVIW VGI |
| 遺伝子ID: | 479 |
| Uniprot: | P54707 |
| セルラーロケーション: | Membrane, Multi-pass membrane protein |
| 計算された分子量: | 115kDa/116kDa |
| 観察された分子量: | 116KDa |
| 同義語: | ATP12A, ATP1AL1, HK |
| バックグラウンド: | The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ATP12A: Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transporter; Energy Metabolism - oxidative phosphorylation; Transporter, ion channel; EC 3.6.3.10; Membrane protein, integral; Membrane protein, multi-pass; Hydrolase Chromosomal Location of Human Ortholog: 13q12.12|13q12.1-q12.3 Cellular Component: hydrogen:potassium-exchanging ATPase complex; intracellular membrane-bound organelle; plasma membrane Molecular Function:hydrogen:potassium-exchanging ATPase activity; sodium:potassium-exchanging ATPase activity Biological Process: ATP hydrolysis coupled proton transport; cellular potassium ion homeostasis; cellular sodium ion homeostasis; potassium ion import |
| NCBI Summary: | The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P54707 |
| NCBI GenInfo Identifier: | 212287925 |
| NCBI Gene ID: | 479 |
| NCBI Accession: | P54707.3 |
| UniProt Secondary Accession: | P54707,Q13816, Q13817, Q16734, Q5W035, Q8N5U2, |
| UniProt Related Accession: | P54707 |
| Molecular Weight: | 116,053 Da |
| NCBI Full Name: | Potassium-transporting ATPase alpha chain 2 |
| NCBI Synonym Full Names: | ATPase H+/K+ transporting non-gastric alpha2 subunit |
| NCBI Official Symbol: | ATP12A |
| NCBI Official Synonym Symbols: | HK; #945; ATP1AL1 |
| NCBI Protein Information: | potassium-transporting ATPase alpha chain 2 |
| UniProt Protein Name: | Potassium-transporting ATPase alpha chain 2 |
| UniProt Synonym Protein Names: | Non-gastric H(+)/K(+) ATPase subunit alpha; Proton pump |
| Protein Family: | Potassium-transporting ATPase |
| UniProt Gene Name: | ATP12A |
| UniProt Entry Name: | AT12A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATP12A antibody (CAB14372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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