Anti-ATG16L1 Antibody (CAB11969)
- SKU:
- CAB11969
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| 抗体名: | Anti-ATG16L1 Antibody |
| 抗体コード: | CAB11969 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ATG16L1 (NP_110430.5). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HT-29, MCF7, HeLa |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ATG16L1 (NP_110430.5). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QEAN RLNA ENEK DSRR RQAR LQKE LAEA AKEP LPVE QDDD IEVI VDET SDHT EETS PVRA ISRA ATKR LSQP AGGL LDSI TNIF GRRS VSSF PVPQ DNVD T |
| 遺伝子ID: | 55054 |
| Uniprot: | Q676U5 |
| セルラーロケーション: | Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane |
| 計算された分子量: | 47kDa/49kDa/53kDa/66kDa/68kDa |
| 観察された分子量: | 72kDa |
| 同義語: | ATG16L1, APG16L, ATG16A, ATG16L, IBD10, WDR30 |
| バックグラウンド: | The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ATG16L1: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, peripheral; Adaptor/scaffold; Autophagy Chromosomal Location of Human Ortholog: 2q37.1 Cellular Component: autophagic vacuole; axoneme; cytosol Molecular Function:identical protein binding; protein binding; small conjugating protein ligase activity Biological Process: autophagic vacuole formation; macroautophagy Disease: Inflammatory Bowel Disease 10 |
| NCBI Summary: | The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010] |
| UniProt Code: | Q676U5 |
| NCBI GenInfo Identifier: | 62510482 |
| NCBI Gene ID: | 55054 |
| NCBI Accession: | Q676U5.2 |
| UniProt Secondary Accession: | Q676U5,Q6IPN1, Q6UXW4, Q6ZVZ5, Q8NCY2, Q96JV5, Q9H619 A3EXK9, A3EXL0, B6ZDH0, |
| UniProt Related Accession: | Q676U5 |
| Molecular Weight: | 49,486 Da |
| NCBI Full Name: | Autophagy-related protein 16-1 |
| NCBI Synonym Full Names: | autophagy related 16 like 1 |
| NCBI Official Symbol: | ATG16L1 |
| NCBI Official Synonym Symbols: | IBD10; WDR30; APG16L; ATG16A; ATG16L |
| NCBI Protein Information: | autophagy-related protein 16-1 |
| UniProt Protein Name: | Autophagy-related protein 16-1 |
| UniProt Synonym Protein Names: | APG16-like 1 |
| Protein Family: | Autophagy-related protein |
| UniProt Gene Name: | ATG16L1 |
| UniProt Entry Name: | A16L1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATG16L1 antibody (CAB11969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunofluorescence analysis of H9C2 cells using ATG16L1 antibody (CAB11969) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using ATG16L1 antibody (CAB11969) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using ATG16L1 antibody (CAB11969) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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