Anti-ATG13 Antibody (CAB0690)
- SKU:
- CAB0690
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| 抗体名: | Anti-ATG13 Antibody |
| 抗体コード: | CAB0690 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 281-480 of human ATG13 (NP_055556.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, A-549, NCI-H460, SH-SY5Y, Rat brain, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 281-480 of human ATG13 (NP_055556.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VHGT QADQ ERLA TCTP SDRT HCAA TPSS SEDT ETVS NSSE GRAS PHDV LETI FVRK VGAF VNKP INQV TLTS LDIP FAMF APKN LELE DTDP MVNP PDSP ETES PLQG SLHS DGSS GGSS GNTH DDFV MIDF KPAF SKDD ILPM DLGT FYRE FQNP PQLS SLSI DIGA QSMA EDLD SLPE KLAV HEKN VREF DAFV ETLQ |
| 遺伝子ID: | 9776 |
| Uniprot: | O75143 |
| セルラーロケーション: | Cytoplasm, Preautophagosomal structure, cytosol |
| 計算された分子量: | 44kDa/52kDa/56kDa/60kDa |
| 観察された分子量: | 75kDa |
| 同義語: | KIAA0652, PARATARG8, ATG13 |
| バックグラウンド: | The protein encoded by this gene is an autophagy factor and a target of the TOR kinase signaling pathway. The encoded protein is essential for autophagosome formation and mitophagy. |
| UniProt Protein Function: | ATG13: an autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. Phosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Part of a complex consisting of ATG13, ULK1 and RB1CC1. Interacts with ATG101. Interacts with ULK1 and -2 via C-terminus. Three isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle; Autophagy Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: mitochondrion; cytosol Molecular Function:protein binding; protein kinase binding Biological Process: autophagic vacuole formation |
| NCBI Summary: | The protein encoded by this gene is an autophagy factor and a target of the TOR kinase signaling pathway. The encoded protein is essential for autophagosome formation and mitophagy. [provided by RefSeq, Oct 2016] |
| UniProt Code: | O75143 |
| NCBI GenInfo Identifier: | 20140441 |
| NCBI Gene ID: | 9776 |
| NCBI Accession: | O75143.1 |
| UniProt Related Accession: | O75143 |
| Molecular Weight: | Observed: 57 kDaPredicted: 57 kDa |
| NCBI Full Name: | Autophagy-related protein 13 |
| NCBI Synonym Full Names: | autophagy related 13 |
| NCBI Official Symbol: | ATG13 |
| NCBI Official Synonym Symbols: | KIAA0652; PARATARG8 |
| NCBI Protein Information: | autophagy-related protein 13 |
| UniProt Protein Name: | Autophagy-related protein 13 |
| Protein Family: | Autophagy protein |
| UniProt Gene Name: | ATG13 |
| UniProt Entry Name: | ATG13_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATG13 antibody (CAB0690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibody (CAB0690) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibody (CAB0690) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using ATG13 antibody (CAB0690). Blue: DAPI for nuclear staining. |
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