Anti-ATG10 Antibody (CAB7390)
- SKU:
- CAB7390
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| 抗体名: | Anti-ATG10 Antibody |
| 抗体コード: | CAB7390 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ATG10 (NP_113670.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse small intestine |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ATG10 (NP_113670.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEED EFIG EKTF QRYC AEFI KHSQ QIGD SWEW RPSK DCSD GYMC KIHF QIKN GSVM SHLG ASTH GQTC LPME EAFE LPLD DCEV IETA AASE VIKY EYHV LYSC SYQV PVLY FRAS FLDG RPLT LKDI WEGV HECY KMRL LQGP WDTI TQQE HPIL GQPF FVLH PCKT NEFM TPVL KNSQ KINK NVNY ITSW LSIV GPVV GLNL PLSY AKAT SQDE RNVP |
| 遺伝子ID: | 83734 |
| Uniprot: | Q9H0Y0 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 14kDa/25kDa |
| 観察された分子量: | 25kDa |
| 同義語: | ATG10, APG10, APG10L, pp12616 |
| バックグラウンド: |
| UniProt Protein Function: | ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; Autophagy; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: cytosol; intracellular Molecular Function:APG12 conjugating enzyme activity Biological Process: autophagy; macroautophagy; positive regulation of protein modification process; protein amino acid lipidation; protein modification by small protein conjugation |
| NCBI Summary: | Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9H0Y0 |
| NCBI GenInfo Identifier: | 62286633 |
| NCBI Gene ID: | 83734 |
| NCBI Accession: | Q9H0Y0.1 |
| UniProt Secondary Accession: | Q9H0Y0,Q6PIX1, Q9H842, B2RE09, |
| UniProt Related Accession: | Q9H0Y0 |
| Molecular Weight: | 14,217 Da |
| NCBI Full Name: | Ubiquitin-like-conjugating enzyme ATG10 |
| NCBI Synonym Full Names: | autophagy related 10 |
| NCBI Official Symbol: | ATG10 |
| NCBI Official Synonym Symbols: | APG10; APG10L; pp12616 |
| NCBI Protein Information: | ubiquitin-like-conjugating enzyme ATG10 |
| UniProt Protein Name: | Ubiquitin-like-conjugating enzyme ATG10 |
| UniProt Synonym Protein Names: | Autophagy-related protein 10; APG10-like |
| Protein Family: | Ubiquitin-like-conjugating enzyme |
| UniProt Gene Name: | ATG10 |
| UniProt Entry Name: | ATG10_HUMAN |
 | Western blot analysis of extracts of mouse small intestine, using ATG10 antibody (CAB7390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Human colon using ATG10 Rabbit pAb (CAB7390) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse spleen using ATG10 Rabbit pAb (CAB7390) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat testis using ATG10 Rabbit pAb (CAB7390) at dilution of 1:100 (40x lens). |
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