Anti-ASPA Antibody (CAB7271)
- SKU:
- CAB7271
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ASPA Antibody |
| 抗体コード: | CAB7271 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human ASPA (NP_001121557.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, HeLa, Mouse brain, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human ASPA (NP_001121557.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MTSC HIAE EHIQ KVAI FGGT HGNE LTGV FLVK HWLE NGAE IQRT GLEV KPFI TNPR AVKK CTRY IDCD LNRI FDLE NLGK KMSE DLPY EVRR AQEI NHLF GPKD SEDS YDII FDLH NTTS NMGC TLIL EDSR NNFL IQMF HYIK TSLA PLPC YVYL IEHP SLKY ATTR SIAK YPVG IEVG PQPQ GVLR ADIL DQMR KMIK HALD FIHH FNEG KEFP PCAI EVYK IIEK VDYP RDEN GEIA AIIH PNLQ DQDW KPLH PGDP MFLT LDGK TIPL GGDC TVYP VFVN EAAY YEKK EAFA KTTK LTLN AKSI RCCL H |
| 遺伝子ID: | 443 |
| Uniprot: | P45381 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 35kDa |
| 観察された分子量: | 36kDa |
| 同義語: | ASPA, ACY2, ASP |
| バックグラウンド: | This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | ASPA: Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids. Defects in ASPA are the cause of Canavan disease (CAND); also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average. Belongs to the AspA/AstE family. Aspartoacylase subfamily. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - histidine; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 3.5.1.15; Hydrolase Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; metal ion binding; hydrolase activity, acting on ester bonds; aspartoacylase activity; aminoacylase activity Biological Process: myelination in the central nervous system; aspartate catabolic process; positive regulation of oligodendrocyte differentiation Disease: Canavan Disease |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P45381 |
| NCBI GenInfo Identifier: | 1168340 |
| NCBI Gene ID: | 443 |
| NCBI Accession: | P45381.1 |
| UniProt Related Accession: | P45381 |
| Molecular Weight: | 313 |
| NCBI Full Name: | Aspartoacylase |
| NCBI Synonym Full Names: | aspartoacylase |
| NCBI Official Symbol: | ASPA |
| NCBI Official Synonym Symbols: | ASP; ACY2 |
| NCBI Protein Information: | aspartoacylase; ACY-2; aminoacylase 2; aminoacylase-2 |
| UniProt Protein Name: | Aspartoacylase |
| UniProt Synonym Protein Names: | Aminoacylase-2; ACY-2 |
| Protein Family: | Aspartocin |
| UniProt Gene Name: | ASPA |
| UniProt Entry Name: | ACY2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ASPA Antibody (CAB7271) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of MCF-7 cells using ASPA antibody (CAB7271). Blue: DAPI for nuclear staining. |
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