Anti-ARL1 Antibody (CAB15254)
- SKU:
- CAB15254
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ARL1 Antibody |
| 抗体コード: | CAB15254 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 62-181 of human ARL1 (NP_001168.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, A-549, Mouse thymus, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 62-181 of human ARL1 (NP_001168.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | KFQV WDLG GQTS IRPY WRCY YSNT DAVI YVVD SCDR DRIG ISKS ELVA MLEE EELR KAIL VVFA NKQD MEQA MTSS EMAN SLGL PALK DRKW QIFK TSAT KGTG LDEA MEWL VETL KSRQ |
| 遺伝子ID: | 400 |
| Uniprot: | P40616 |
| セルラーロケーション: | Cytoplasmic side, Golgi apparatus membrane, Lipid-anchor, Membrane, Peripheral membrane protein |
| 計算された分子量: | 18kDa/20kDa |
| 観察された分子量: | 20kDa |
| 同義語: | ARL1, ARFL1 |
| バックグラウンド: | The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | ARL1: GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric; G protein, monomeric, ARF Chromosomal Location of Human Ortholog: 12q23.2 Cellular Component: Golgi membrane; Golgi apparatus; cytoplasm; trans-Golgi network Molecular Function:GTPase activity; protein domain specific binding; GTP binding; metal ion binding; enzyme activator activity Biological Process: phospholipase D activation; toxin metabolic process; protein localization in Golgi apparatus; small GTPase mediated signal transduction; retrograde transport, endosome to Golgi; Golgi organization and biogenesis; Golgi vesicle transport |
| NCBI Summary: | The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P40616 |
| NCBI GenInfo Identifier: | 728888 |
| NCBI Gene ID: | 400 |
| NCBI Accession: | P40616.1 |
| UniProt Related Accession: | P40616 |
| Molecular Weight: | 20kDa |
| NCBI Full Name: | ADP-ribosylation factor-like protein 1 |
| NCBI Synonym Full Names: | ADP ribosylation factor like GTPase 1 |
| NCBI Official Symbol: | ARL1 |
| NCBI Official Synonym Symbols: | ARFL1 |
| NCBI Protein Information: | ADP-ribosylation factor-like protein 1 |
| UniProt Protein Name: | ADP-ribosylation factor-like protein 1 |
| Protein Family: | ARL14 effector protein |
| UniProt Gene Name: | ARL1 |
| UniProt Entry Name: | ARL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARL1 antibody (CAB15254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunofluorescence analysis of C6 cells using ARL1 Polyclonal Antibody (CAB15254) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using ARL1 Polyclonal Antibody (CAB15254) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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