Anti-ARHGEF9 Antibody (CAB7964)
- SKU:
- CAB7964
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | ARHGEF9 Rabbit Polyclonal Antibody |
| 抗体コード: | CAB7964 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 307-516 of human ARHGEF9 (NP_056000.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | BT-474, 22Rv1, HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 307-516 of human ARHGEF9 (NP_056000.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | WEGE DILD RSSE LIYT GEMA WIYQ PYGR NQQR VFFL FDHQ MVLC KKDL IRRD ILYY KGRI DMDK YEVV DIED GRDD DFNV SMKN AFKL HNKE TEEI HLFF AKKL EEKI RWLR AFRE ERKM VQED EKIG FEIS ENQK RQAA MTVR KVPK QKGV NSAR SVPP SYPP PQDP LNHG QYLV PDGI AQSQ VFEF TEPK RSQS PFWQ NFSR LTPF KK |
| 遺伝子ID: | 23229 |
| Uniprot: | O43307 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 49kDa/54kDa/60kDa |
| 観察された分子量: | 61kDa |
| 同義語: | ARHGEF9, COLLYBISTIN, EIEE8, HPEM-2, PEM-2, PEM2 |
| バックグラウンド: | The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ARHGEF9: Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters. Defects in ARHGEF9 are the cause of pileptic encephalopathy, early infantile, type 8 (EIEE8). A disorder characterized by hyperekplexia and early infantile epileptic encephalopathy. Neurologic features include exaggerated startle response, seizures, impaired psychomotor development, and mental retardation. Seizures can be provoked by tactile stimulation or extreme emotion. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GAPs; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: Xq11.1 Cellular Component: cytoplasm; cytosol Molecular Function:guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity Biological Process: positive regulation of apoptosis; regulation of small GTPase mediated signal transduction Disease: Epileptic Encephalopathy, Early Infantile, 8 |
| NCBI Summary: | The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] |
| UniProt Code: | O43307 |
| NCBI GenInfo Identifier: | 111599692 |
| NCBI Gene ID: | 23229 |
| NCBI Accession: | BC117406 |
| UniProt Secondary Accession: | O43307,Q5JSL6, A8K1S8, B4DHC7, F8W7P8, |
| UniProt Related Accession: | O43307 |
| Molecular Weight: | 49,458 Da |
| NCBI Full Name: | Homo sapiens Cdc42 guanine nucleotide exchange factor (GEF) 9, mRNA |
| NCBI Synonym Full Names: | Cdc42 guanine nucleotide exchange factor 9 |
| NCBI Official Symbol: | ARHGEF9 |
| NCBI Official Synonym Symbols: | PEM2; EIEE8; PEM-2; HPEM-2; COLLYBISTIN |
| NCBI Protein Information: | rho guanine nucleotide exchange factor 9 |
| UniProt Protein Name: | Rho guanine nucleotide exchange factor 9 |
| UniProt Synonym Protein Names: | Collybistin; PEM-2 homolog; Rac/Cdc42 guanine nucleotide exchange factor 9 |
| Protein Family: | Rho guanine nucleotide exchange factor |
| UniProt Gene Name: | ARHGEF9 |
| UniProt Entry Name: | ARHG9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARHGEF9 antibody (CAB7964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse heart using ARHGEF9 antibody (CAB7964) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using ARHGEF9 antibody (CAB7964). Blue: DAPI for nuclear staining. |
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