Anti-ARHGDIB Antibody (CAB3740)
- SKU:
- CAB3740
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ARHGDIB Antibody |
| 抗体コード: | CAB3740 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human ARHGDIB (NP_001166.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Raji, HL-60, HT-1080, Mouse brain, Mouse lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human ARHGDIB (NP_001166.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MTEK APEP HVEE DDDD ELDS KLNY KPPP QKSL KELQ EMDK DDES LIKY KKTL LGDG PVVT DPKA PNVV VTRL TLVC ESAP GPIT MDLT GDLE ALKK ETIV LKEG SEYR VKIH FKVN RDIV SGLK YVQH TYRT GVKV DKAT FMVG SYGP RPEE YEFL TPVE EAPK GMLA RGTY HNKS FFTD DDKQ DHLS WEWN LSIK KEWT E |
| 遺伝子ID: | 397 |
| Uniprot: | P52566 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 22kDa |
| 観察された分子量: | 23kDa |
| 同義語: | ARHGDIB, D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2 |
| バックグラウンド: |
| UniProt Protein Function: | RhoGDI beta: regulates the GDP/GTP exchange reaction of Rho proteins by inhibiting the dissociation of GDP from them, keeping these small GTPases in an inactive state. The GDIs are made up of two domains: a flexible N-terminal domain of about 70 amino acid residues and a folded 134-residue C-terminal domain. Regulates the GTP/GDP cycling of glucose-responsive small GTPases. Its phosphorylation on Y156, S101, and S174 mediates the cycling of Cdc42 and Rac1 to regulate second-phase insulin secretion. Interacts with WNK1. |
| UniProt Protein Details: | Protein type:G protein regulator, misc.; Cell adhesion; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12p12.3 Cellular Component: cytoskeleton; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol Molecular Function:Rho GDP-dissociation inhibitor activity; GTPase activator activity Biological Process: regulation of small GTPase mediated signal transduction; regulation of catalytic activity; small GTPase mediated signal transduction; multicellular organismal development; immune response; negative regulation of cell adhesion; cell motility; actin cytoskeleton organization and biogenesis; Rho protein signal transduction; positive regulation of GTPase activity |
| NCBI Summary: | Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM, Dec 2010] |
| UniProt Code: | P52566 |
| NCBI GenInfo Identifier: | 1707893 |
| NCBI Gene ID: | 397 |
| NCBI Accession: | P52566.3 |
| UniProt Secondary Accession: | P52566,B5BU79, |
| UniProt Related Accession: | P52566 |
| Molecular Weight: | 201 |
| NCBI Full Name: | Rho GDP-dissociation inhibitor 2 |
| NCBI Synonym Full Names: | Rho GDP dissociation inhibitor (GDI) beta |
| NCBI Official Symbol: | ARHGDIB |
| NCBI Official Synonym Symbols: | D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2 |
| NCBI Protein Information: | rho GDP-dissociation inhibitor 2; Rho GDI 2; rho-GDI beta |
| UniProt Protein Name: | Rho GDP-dissociation inhibitor 2 |
| UniProt Synonym Protein Names: | Ly-GDI; Rho-GDI beta |
| Protein Family: | Rho GDP-dissociation inhibitor |
| UniProt Gene Name: | ARHGDIB |
| UniProt Entry Name: | GDIR2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARHGDIB antibody (CAB3740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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