Anti-ARHGAP5 Antibody (CAB3587)
- SKU:
- CAB3587
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ARHGAP5 Antibody |
| 抗体コード: | CAB3587 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, Jurkat, 293T, HepG2, PC-12, Mouse brain, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EGLY RVSG NKTD QDNI QKQF DQDH NINL VSME VTVN AVAG ALKA FFAD LPDP LIPY SLHP ELLE AAKI PDKT ERLH ALKE IVKK FHPV NYDV FRYV ITHL NRVS QQHK INLM TADN LSIC FWPT LMRP DFEN REFL STTK IHQS VVET FIQQ CQFF FYNG EIVE TTNI VAPP PPSN PGQL VEPM VPLQ LPPP LQPQ LIQP QLQT DPLG II |
| 遺伝子ID: | 394 |
| Uniprot: | Q13017 |
| セルラーロケーション: | Cytoplasm, Membrane, Peripheral membrane protein |
| 計算された分子量: | 26kDa/27kDa/172kDa |
| 観察された分子量: | 185kDa |
| 同義語: | ARHGAP5, GFI2, RhoGAP5, p190-B, p190BRhoGAP |
| バックグラウンド: | Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ARHGAP5: a GTPase activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. Negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. |
| UniProt Protein Details: | Protein type:GAPs; Motility/polarity/chemotaxis; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: 14q12 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; SH2 domain binding Biological Process: regulation of small GTPase mediated signal transduction; mammary gland development; metabolic process; small GTPase mediated signal transduction; positive regulation of mesenchymal cell proliferation; cell adhesion; regulation of cell size; Rho protein signal transduction; positive regulation of cell migration |
| NCBI Summary: | Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13017 |
| NCBI GenInfo Identifier: | 190358871 |
| NCBI Gene ID: | 394 |
| NCBI Accession: | Q13017.2 |
| UniProt Related Accession: | Q13017 |
| Molecular Weight: | |
| NCBI Full Name: | Rho GTPase-activating protein 5 |
| NCBI Synonym Full Names: | Rho GTPase activating protein 5 |
| NCBI Official Symbol: | ARHGAP5 |
| NCBI Official Synonym Symbols: | GFI2; p190-B; RhoGAP5; p190BRhoGAP |
| NCBI Protein Information: | rho GTPase-activating protein 5 |
| UniProt Protein Name: | Rho GTPase-activating protein 5 |
| UniProt Synonym Protein Names: | Rho-type GTPase-activating protein 5; p190-B |
| UniProt Gene Name: | ARHGAP5 |
| UniProt Entry Name: | RHG05_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARHGAP5 antibody (CAB3587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using ARHGAP5 antibody (CAB3587) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using ARHGAP5 antibody (CAB3587) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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