Anti-ARFGAP3 Antibody (CAB8204)
- SKU:
- CAB8204
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | ARFGAP3 Rabbit Polyclonal Antibody |
| 抗体コード: | CAB8204 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 217-516 of human ARFGAP3 (NP_055385.3). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HepG2, Mouse pancreas |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 217-516 of human ARFGAP3 (NP_055385.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | KPNQ AKKG LGAK KGSL GAQK LANT CFNE IEKQ AQAA DKMK EQED LAKV VSKE ESIV SSLR LAYK DLEI QMKK DEKM NISG KKNV DSDR LGMG FGNC RSVI SHSV TSDM QTIE QESP IMAK PRKK YNDD SDDS YFTS SSSY FDEP VELR SSSF SSWD DSSD SYWK KETS KDTE TVLK TTGY SDRP TARR KPDY EPVE NTDE AQKK FGNV KAIS SDMY FGRQ SQAD YETR ARLE RLSA SSSI SSAD LFEE PRKQ PAGN YSLS SVLP NAPD MAQF KQGV RSVA GKLS VFAN GVVT SIQD RYGS |
| 遺伝子ID: | 26286 |
| Uniprot: | Q9NP61 |
| セルラーロケーション: | Cytoplasm, Cytoplasmic side, Golgi apparatus membrane, Peripheral membrane protein |
| 計算された分子量: | 51kDa/56kDa |
| 観察された分子量: | 57kDa |
| 同義語: | ARFGAP3, ARFGAP1 |
| バックグラウンド: | The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. |
| UniProt Protein Function: | GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. |
| NCBI Summary: | The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008] |
| UniProt Code: | Q9NP61 |
| NCBI GenInfo Identifier: | 21263420 |
| NCBI Gene ID: | 26286 |
| NCBI Accession: | Q9NP61.1 |
| UniProt Secondary Accession: | Q9NP61,Q9BSC6, Q9H9J0, Q9NT10, Q9NUP5, Q9Y4V3, Q9Y4V4 E9PB03, |
| UniProt Related Accession: | Q9NP61 |
| Molecular Weight: | 51,990 Da |
| NCBI Full Name: | ADP-ribosylation factor GTPase-activating protein 3 |
| NCBI Synonym Full Names: | ADP ribosylation factor GTPase activating protein 3 |
| NCBI Official Symbol: | ARFGAP3 |
| NCBI Official Synonym Symbols: | ARFGAP1 |
| NCBI Protein Information: | ADP-ribosylation factor GTPase-activating protein 3 |
| UniProt Protein Name: | ADP-ribosylation factor GTPase-activating protein 3 |
| Protein Family: | ADP-ribosylation factor GTPase-activating protein |
| UniProt Gene Name: | ARFGAP3 |
| UniProt Entry Name: | ARFG3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARFGAP3 antibody (CAB8204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse stomach using ARFGAP3 antibody (CAB8204) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using ARFGAP3 antibody (CAB8204) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophagus using ARFGAP3 antibody (CAB8204) at dilution of 1:100 (40x lens). |
View AllClose