Anti-ARF5 Antibody (CAB3737)
- SKU:
- CAB3737
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ARF5 Antibody |
| 抗体コード: | CAB3737 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF5 (NP_001653.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, A375, Mouse liver, Mouse brain, Mouse spleen, Mouse kidney, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF5 (NP_001653.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGLT VSAL FSRI FGKK QMRI LMVG LDAA GKTT ILYK LKLG EIVT TIPT IGFN VETV EYKN ICFT VWDV GGQD KIRP LWRH YFQN TQGL IFVV DSND RERV QESA DELQ KMLQ EDEL RDAV LLVF ANKQ DMPN AMPV SELT DKLG LQHL RSRT WYVQ ATCA TQGT GLYD GLDW LSHE LSKR |
| 遺伝子ID: | 381 |
| Uniprot: | P84085 |
| セルラーロケーション: | Cytoplasm, Golgi apparatus, Lipid-anchor, Membrane, perinuclear region |
| 計算された分子量: | 20kDa |
| 観察された分子量: | 16kDa |
| 同義語: | ARF5 |
| バックグラウンド: | This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. |
| UniProt Protein Function: | ARF5: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, ARF; G protein; G protein, monomeric; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 7q31.3 Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; plasma membrane Molecular Function:GTPase activity; protein binding; GTP binding Biological Process: vesicle-mediated transport; protein transport; metabolic process; small GTPase mediated signal transduction |
| NCBI Summary: | This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010] |
| UniProt Code: | P84085 |
| NCBI GenInfo Identifier: | 51316990 |
| NCBI Gene ID: | 381 |
| NCBI Accession: | P84085.2 |
| UniProt Secondary Accession: | P84085,P26437, |
| UniProt Related Accession: | P84085 |
| Molecular Weight: | 180 |
| NCBI Full Name: | ADP-ribosylation factor 5 |
| NCBI Synonym Full Names: | ADP-ribosylation factor 5 |
| NCBI Official Symbol: | ARF5 |
| NCBI Protein Information: | ADP-ribosylation factor 5 |
| UniProt Protein Name: | ADP-ribosylation factor 5 |
| Protein Family: | Auxin response factor |
| UniProt Gene Name: | ARF5 |
| UniProt Entry Name: | ARF5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARF5 antibody (CAB3737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of HeLa cells using ARF5 antibody (CAB3737) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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