Anti-ARF4 Antibody (CAB7644)
- SKU:
- CAB7644
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ARF4 Antibody |
| 抗体コード: | CAB7644 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF4 (NP_001651.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SH-SY5Y, Mouse brain, Mouse testis, Rat testis, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF4 (NP_001651.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGLT ISSL FSRL FGKK QMRI LMVG LDAA GKTT ILYK LKLG EIVT TIPT IGFN VETV EYKN ICFT VWDV GGQD RIRP LWKH YFQN TQGL IFVV DSND RERI QEVA DELQ KMLL VDEL RDAV LLLF ANKQ DLPN AMAI SEMT DKLG LQSL RNRT WYVQ ATCA TQGT GLYE GLDW LSNE LSKR |
| 遺伝子ID: | 378 |
| Uniprot: | P18085 |
| セルラーロケーション: | Golgi apparatus, Lipid-anchor, Membrane |
| 計算された分子量: | 20kDa |
| 観察された分子量: | 14kDa |
| 同義語: | ARF4, ARF2 |
| バックグラウンド: | This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. |
| UniProt Protein Function: | ARF4: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, ARF; G protein Chromosomal Location of Human Ortholog: 3p14.3 Cellular Component: cytosol; dendritic spine; membrane Molecular Function:ARF guanyl-nucleotide exchange factor activity; epidermal growth factor receptor binding; GTP binding; GTPase activity; protein binding Biological Process: cell migration; ER to Golgi vesicle-mediated transport; negative regulation of apoptosis; phospholipase D activation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid ADP-ribosylation; retrograde vesicle-mediated transport, Golgi to ER |
| NCBI Summary: | This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P18085 |
| NCBI GenInfo Identifier: | 114123 |
| NCBI Gene ID: | 378 |
| NCBI Accession: | P18085.3 |
| UniProt Secondary Accession: | P18085,P21371, B2R7J7, |
| UniProt Related Accession: | P18085 |
| Molecular Weight: | 20511 Da |
| NCBI Full Name: | ADP-ribosylation factor 4 |
| NCBI Synonym Full Names: | ADP ribosylation factor 4 |
| NCBI Official Symbol: | ARF4 |
| NCBI Official Synonym Symbols: | ARF2 |
| NCBI Protein Information: | ADP-ribosylation factor 4 |
| UniProt Protein Name: | ADP-ribosylation factor 4 |
| Protein Family: | Auxin response factor |
| UniProt Gene Name: | ARF4 |
| UniProt Entry Name: | ARF4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARF4 antibody (CAB7644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using ARF4 Rabbit pAb (CAB7644) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using ARF4 Rabbit pAb (CAB7644) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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