Anti-ARF1 Antibody (CAB2821)
- SKU:
- CAB2821
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ARF1 Antibody |
| 抗体コード: | CAB2821 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide of human ARF1 |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | BT-474, HT-29, SKOV3, MCF7, HeLa, Mouse lung, Mouse brain, Mouse heart, Rat liver |
| 免疫原: | A synthetic peptide of human ARF1 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 375 |
| Uniprot: | P84077 |
| セルラーロケーション: | Cell junction, Cytoplasm, Golgi apparatus, Lipid-anchor, Membrane, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse, synaptosome |
| 計算された分子量: | 20kDa |
| 観察された分子量: | 17kDa |
| 同義語: | ARF1 |
| バックグラウンド: | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric Chromosomal Location of Human Ortholog: 1q42 Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; receptor signaling protein activity Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus |
| NCBI Summary: | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P84077 |
| NCBI GenInfo Identifier: | 51316985 |
| NCBI Gene ID: | 375 |
| NCBI Accession: | P84077.2 |
| UniProt Secondary Accession: | P84077,P10947, P32889, |
| UniProt Related Accession: | P84077 |
| Molecular Weight: | 181 |
| NCBI Full Name: | ADP-ribosylation factor 1 |
| NCBI Synonym Full Names: | ADP-ribosylation factor 1 |
| NCBI Official Symbol: | ARF1 |
| NCBI Protein Information: | ADP-ribosylation factor 1 |
| UniProt Protein Name: | ADP-ribosylation factor 1 |
| Protein Family: | Auxin response factor |
| UniProt Gene Name: | ARF1 |
| UniProt Entry Name: | ARF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARF1 antibody (CAB2821) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using ARF1 antibody (CAB2821) (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using ARF1 antibody (CAB2821) (40x lens). |
 | Immunohistochemistry of paraffin-embedded human prostate using ARF1 antibody (CAB2821) (40x lens). |
 | Immunofluorescence analysis of HeLa cells using ARF1 antibody (CAB2821) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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