Anti-AP2A2 Antibody (CAB5391)
- SKU:
- CAB5391
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-AP2A2 Antibody |
| 抗体コード: | CAB5391 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 590-939 of human AP2A2 (NP_036437.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 22Rv1, SKOV3, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 590-939 of human AP2A2 (NP_036437.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | STDI LATV LEEM PPFP ERES SILA KLKK KKGP STVT DLED TKRD RSVD VNGG PEPA PAST SAVS TPSP SADL LGLG AAPP APAG PPPS SGGS GLLV DVFS DSAS VVAP LAPG SEDN FARF VCKN NGVL FENQ LLQI GLKS EFRQ NLGR MFIF YGNK TSTQ FLNF TPTL ICSD DLQP NLNL QTKP VDPT VEGG AQVQ QVVN IECV SDFT EAPV LNIQ FRYG GTFQ NVSV QLPI TLNK FFQP TEMA SQDF FQRW KQLS NPQQ EVQN IFKA KHPM DTEV TKAK IIGF GSAL LEEV DPNP ANFV GAGI IHTK TTQI GCLL RLEP NLQA QMYR LTLR TSKE AVSQ RLCE LLSA QF |
| 遺伝子ID: | 161 |
| Uniprot: | O94973 |
| セルラーロケーション: | Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit |
| 計算された分子量: | 73kDa/103kDa/104kDa |
| 観察された分子量: | 118kDa |
| 同義語: | AP2A2, ADTAB, CLAPA2, HIP-9, HIP9, HYPJ |
| バックグラウンド: | The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. |
| UniProt Protein Function: | AP2A2: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C- terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes large subunit family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Vesicle Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: cytoplasmic membrane-bound vesicle; plasma membrane; AP-2 adaptor complex; cytosol Molecular Function:protein binding; lipid binding; protein kinase binding; protein transporter activity Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; axon guidance; intracellular protein transport; synaptic transmission; nerve growth factor receptor signaling pathway; viral reproduction; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus |
| UniProt Code: | O94973 |
| NCBI GenInfo Identifier: | 12643300 |
| NCBI Gene ID: | 161 |
| NCBI Accession: | O94973.2 |
| UniProt Secondary Accession: | O94973,O75403, Q53ET1, Q96SI8, |
| UniProt Related Accession: | O94973 |
| Molecular Weight: | |
| NCBI Full Name: | AP-2 complex subunit alpha-2 |
| NCBI Synonym Full Names: | adaptor-related protein complex 2, alpha 2 subunit |
| NCBI Official Symbol: | AP2A2 |
| NCBI Official Synonym Symbols: | HIP9; HYPJ; ADTAB; HIP-9; CLAPA2 |
| NCBI Protein Information: | AP-2 complex subunit alpha-2; 100 kDa coated vesicle protein C; adapter-related protein complex 2 subunit alpha-2; adaptin, alpha B; adaptor-related protein complex 2 subunit alpha-2; alpha-adaptin C; Huntingtin interacting protein J; alpha2-adaptin; clathrin assembly protein complex 2 alpha-C large chain; clathrin-associated/assembly/adaptor protein, large, alpha 2; huntingtin yeast partner J; huntingtin-interacting protein 9; plasma membrane adaptor HA2/AP2 adaptin alpha C subunit |
| UniProt Protein Name: | AP-2 complex subunit alpha-2 |
| UniProt Synonym Protein Names: | 100 kDa coated vesicle protein C; Adaptor protein complex AP-2 subunit alpha-2; Adaptor-related protein complex 2 subunit alpha-2; Alpha-adaptin C; Alpha2-adaptin; Clathrin assembly protein complex 2 alpha-C large chain; Huntingtin yeast partner J; Huntingtin-interacting protein 9; HIP-9; Huntingtin-interacting protein J; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit |
| Protein Family: | AP-2 complex |
| UniProt Gene Name: | AP2A2 |
| UniProt Entry Name: | AP2A2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AP2A2 antibody (CAB5391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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