Anti-ANAPC5 Antibody (CAB7109)
- SKU:
- CAB7109
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-ANAPC5 Antibody |
| 抗体コード: | CAB7109 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2). |
| 申し込み: | WB IHC IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SW620, MCF-7, HL-60, 22Rv1, A-549, Mouse spleen |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MASV HESL YFNP MMTN GVVH ANVF GIKD WVTP YKIA VLVL LNEM SRTG EGAV SLME RRRL NQLL LPLL QGPD ITLS KLYK LIEE SCPQ LANS VQIR IKLM AEGE LKDM EQFF DDLS DSFS GTEP EVHK TSVV GLFL RHMI LAYS KLSF SQVF KLYT ALQQ YFQN GEKK TVED ADME LTSR DEGE RKME KEEL DVSV REEE VSCS GPLS QKQA EFFL SQQA SLLK NDET KALT PASL QKEL NNLL KFNP DF |
| 遺伝子ID: | 51433 |
| Uniprot: | Q9UJX4 |
| セルラーロケーション: | |
| 計算された分子量: | 25kDa/72kDa/85kDa |
| 観察された分子量: | 85kDa |
| 同義語: | ANAPC5, APC5 |
| バックグラウンド: | This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. |
| UniProt Protein Function: | APC5: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC5 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol Molecular Function:protein phosphatase binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle |
| NCBI Summary: | This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008] |
| UniProt Code: | Q9UJX4 |
| NCBI GenInfo Identifier: | 37537861 |
| NCBI Gene ID: | 51433 |
| NCBI Accession: | Q9UJX4.2 |
| UniProt Secondary Accession: | Q9UJX4,Q8N4H7, Q9BQD4, E9PFB2, |
| UniProt Related Accession: | Q9UJX4 |
| Molecular Weight: | 72,526 Da |
| NCBI Full Name: | Anaphase-promoting complex subunit 5 |
| NCBI Synonym Full Names: | anaphase promoting complex subunit 5 |
| NCBI Official Symbol: | ANAPC5 |
| NCBI Official Synonym Symbols: | APC5 |
| NCBI Protein Information: | anaphase-promoting complex subunit 5; cyclosome subunit 5 |
| UniProt Protein Name: | Anaphase-promoting complex subunit 5 |
| UniProt Synonym Protein Names: | Cyclosome subunit 5 |
| UniProt Gene Name: | ANAPC5 |
| UniProt Entry Name: | APC5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ANAPC5 antibody (CAB7109) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using ANAPC5 antibody (CAB7109) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human kidney using ANAPC5 antibody (CAB7109) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ANAPC5 antibody (CAB7109). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (CAB7109) at a dilition of 1:1000. |