Anti-AMACR Antibody (CAB1130)
- SKU:
- CAB1130
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-AMACR Antibody |
| 抗体コード: | CAB1130 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human AMACR (NP_976316.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | LNCap, Mouse kindey, Mouse liver, Rat kidney, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human AMACR (NP_976316.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MALQ GISV VELS GLAP GPFC AMVL ADFG ARVV RVDR PGSR YDVS RLGR GKRS LVLD LKQP RGAA VLRR LCKR SDVL LEPF RRGV MEKL QLGP EILQ RENP RLIY ARLS GFGQ SGSF CRLA GHDI NYLA LSGG RNSI FKFF SVEN SEIE SVGS TSRT EHVG WWST FLYD LQDS RWGI HGCW SNRT PVLR AADQ RTWT KV |
| 遺伝子ID: | 23600 |
| Uniprot: | Q9UHK6 |
| セルラーロケーション: | Mitochondrion, Peroxisome |
| 計算された分子量: | 22kDa/25kDa/42kDa/43kDa |
| 観察された分子量: | 42kDa/43kDa |
| 同義語: | AMACR, AMACRD, CBAS4, P504S, RACE, RM |
| バックグラウンド: | This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. |
| UniProt Protein Function: | AMACR: Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. Belongs to the CaiB/BaiF CoA-transferase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - primary bile acid biosynthesis; EC 5.1.99.4; Isomerase; Mitochondrial Chromosomal Location of Human Ortholog: 5p13 Cellular Component: peroxisomal matrix; mitochondrion; cytoplasm; peroxisome Molecular Function:receptor binding; alpha-methylacyl-CoA racemase activity Biological Process: bile acid biosynthetic process; fatty acid beta-oxidation using acyl-CoA oxidase; bile acid metabolic process; cellular lipid metabolic process Disease: Bile Acid Synthesis Defect, Congenital, 4; Alpha-methylacyl-coa Racemase Deficiency |
| NCBI Summary: | This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011] |
| UniProt Code: | Q9UHK6 |
| NCBI GenInfo Identifier: | 313104070 |
| NCBI Gene ID: | 23600 |
| NCBI Accession: | Q9UHK6.2 |
| UniProt Secondary Accession: | Q9UHK6,O43673, Q3KT79, Q96GH1, Q9Y3Q1, A5YM47, B8Y916 B8Y918, F8W9N1, |
| UniProt Related Accession: | Q9UHK6 |
| Molecular Weight: | 382 |
| NCBI Full Name: | Alpha-methylacyl-CoA racemase |
| NCBI Synonym Full Names: | alpha-methylacyl-CoA racemase |
| NCBI Official Symbol: | AMACR |
| NCBI Official Synonym Symbols: | RM; RACE; CBAS4; AMACRD |
| NCBI Protein Information: | alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase |
| UniProt Protein Name: | Alpha-methylacyl-CoA racemase |
| UniProt Synonym Protein Names: | 2-methylacyl-CoA racemase |
| Protein Family: | Alpha-methylacyl-CoA racemase |
| UniProt Gene Name: | AMACR |
| UniProt Entry Name: | AMACR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AMACR antibody (CAB1130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human prostate cancer using AMACR Rabbit pAb (CAB1130) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat kidney using AMACR Rabbit pAb (CAB1130) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse kidney using AMACR Rabbit pAb (CAB1130) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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