Anti-Alpha-Tubulin Antibody (CABC031)
- SKU:
- CABC031
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Frequently bought together:
Description
| 抗体名: | Anti-Alpha-Tubulin Antibody |
| 抗体コード: | CABC031 |
| 抗体サイズ: | 100 uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human Alpha-Tubulin |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, Jurkat, A-431, Mouse brain, Mouse lung, Rat brain |
| 免疫原: | Recombinant protein of human Alpha-Tubulin |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 7277 |
| Uniprot: | P68366 |
| セルラーロケーション: | Cytoplasm, cytoskeleton |
| 計算された分子量: | 48kDa/49kDa |
| 観察された分子量: | 55kDa |
 | Western blot analysis of extracts of various cell lines, using alpha-Tubulin antibody (CABC031) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
| 同義語: | TUBA4A, ALS22, H2-ALPHA, TUBA1 |
| バックグラウンド: | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | TUBA4A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 2q35 Cellular Component: microtubule; extracellular region; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton Biological Process: protein polymerization; 'de novo' posttranslational protein folding; platelet activation; cellular protein metabolic process; platelet degranulation; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; microtubule-based process Disease: Amyotrophic Lateral Sclerosis 22 With Or Without Frontotemporal Dementia |
| NCBI Summary: | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] |
| UniProt Code: | P68366 |
| NCBI GenInfo Identifier: | 55977476 |
| NCBI Gene ID: | 7277 |
| NCBI Accession: | P68366.1 |
| UniProt Related Accession: | P68366 |
| Molecular Weight: | 50kDa |
| NCBI Full Name: | Tubulin alpha-4A chain |
| NCBI Synonym Full Names: | tubulin alpha 4a |
| NCBI Official Symbol: | TUBA4A |
| NCBI Official Synonym Symbols: | ALS22; TUBA1; H2-ALPHA |
| NCBI Protein Information: | tubulin alpha-4A chain |
| UniProt Protein Name: | Tubulin alpha-4A chain |
| UniProt Synonym Protein Names: | Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain |
| Protein Family: | Tubulin |
| UniProt Gene Name: | TUBA4A |
| UniProt Entry Name: | TBA4A_HUMAN |