Anti-ALG2 Antibody (CAB7843)
- SKU:
- CAB7843
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ALG2 Antibody |
| 抗体コード: | CAB7843 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 197-416 of human ALG2 (NP_149078.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 197-416 of human ALG2 (NP_149078.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DVLY PSLN VTSF DSVV PEKL DDLV PKGK KFLL LSIN RYER KKNL TLAL EALV QLRG RLTS QDWE RVHL IVAG GYDE RVLE NVEH YQEL KKMV QQSD LGQY VTFL RSFS DKQK ISLL HSCT CVLY TPSN EHFG IVPL EAMY MQCP VIAV NSGG PLES IDHS VTGF LCEP DPVH FSEA IEKF IREP SLKA TMGL AGRA RVKE KFSP EAFT EQLY RYVT KLLV |
| 遺伝子ID: | 85365 |
| Uniprot: | Q9H553 |
| セルラーロケーション: | Membrane, Single-pass membrane protein |
| 計算された分子量: | 37kDa/47kDa |
| 観察された分子量: | 47kDa |
| 同義語: | ALG2, CDG1I, CDGIi, CMS14, CMSTA3, NET38, hALPG2, alpha-1, 3/1 |
| バックグラウンド: | This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1, 3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | ALG2: Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)- dolichol diphosphate. Defects in ALG2 are the cause of congenital disorder of glycosylation type 1I (CDG1I). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.4.1.257; Transferase; Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.132 Chromosomal Location of Human Ortholog: 9q22.33 Cellular Component: cytoplasm; endoplasmic reticulum membrane; membrane; nucleus; perinuclear region of cytoplasm Molecular Function:alpha-1,3-mannosyltransferase activity; calcium-dependent protein binding; protein binding; protein heterodimerization activity; protein N-terminus binding Biological Process: dolichol-linked oligosaccharide biosynthetic process; protein amino acid glycosylation in endoplasmic reticulum; response to calcium ion Disease: Congenital Disorder Of Glycosylation, Type Ii; Myasthenic Syndrome, Congenital, With Tubular Aggregates 3 |
| NCBI Summary: | This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008] |
| UniProt Code: | Q9H553 |
| NCBI GenInfo Identifier: | 46395991 |
| NCBI Gene ID: | 85365 |
| NCBI Accession: | Q9H553.1 |
| UniProt Secondary Accession: | Q9H553,Q8NBX2, Q8NC39, A2A2Y0, |
| UniProt Related Accession: | Q9H553 |
| Molecular Weight: | 37,017 Da |
| NCBI Full Name: | Alpha-1,3/1,6-mannosyltransferase ALG2 |
| NCBI Synonym Full Names: | ALG2, alpha-1,3/1,6-mannosyltransferase |
| NCBI Official Symbol: | ALG2 |
| NCBI Official Synonym Symbols: | CDGIi; CMS14; NET38; CMSTA3; hALPG2 |
| NCBI Protein Information: | alpha-1,3/1,6-mannosyltransferase ALG2 |
| UniProt Protein Name: | Alpha-1,3/1,6-mannosyltransferase ALG2 |
| UniProt Synonym Protein Names: | Asparagine-linked glycosylation protein 2 homolog; GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase; GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase; GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase |
| Protein Family: | Alpha-1,3/1,6-mannosyltransferase |
| UniProt Gene Name: | ALG2 |
| UniProt Entry Name: | ALG2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ALG2 antibody (CAB7843) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using ALG2 Antibody (CAB7843) at dilution of 1:100 (40x lens). |
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