Anti-ALG1 Antibody (CAB7818)
- SKU:
- CAB7818
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ALG1 Antibody |
| 抗体コード: | CAB7818 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 215-464 of human ALG1 (NP_061982.3). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, HepG2, Mouse liver, Mouse kidney, Mouse ovary, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 215-464 of human ALG1 (NP_061982.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | YDKP ASFF KETP LDLQ HRLF MKLG SMHS PFRA RSEP EDPV TERS AFTE RDAG SGLV TRLR ERPA LLVS STSW TEDE DFSI LLAA LEKF EQLT LDGH NLPS LVCV ITGK GPLR EYYS RLIH QKHF QHIQ VCTP WLEA EDYP LLLG SADL GVCL HTSS SGLD LPMK VVDM FGCC LPVC AVNF KCLH ELVK HEEN GLVF EDSE ELAA QLQM LFSN FPDP AGKL NQFR KNLR ESQQ LRWD ESWV QTVL PLVM DT |
| 遺伝子ID: | 56052 |
| Uniprot: | Q9BT22 |
| セルラーロケーション: | Endoplasmic reticulum membrane, Single-pass type II membrane protein |
| 計算された分子量: | 40kDa/52kDa |
| 観察された分子量: | 60kDa |
| 同義語: | ALG1, CDG1K, HMAT1, HMT-1, HMT1, MT-1, Mat-1, hMat-1 |
| バックグラウンド: | The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik. |
| UniProt Protein Function: | ALG1: Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER. Defects in ALG1 are the cause of congenital disorder of glycosylation type 1K (CDG1K). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 33 subfamily. |
| UniProt Protein Details: | Protein type:Endoplasmic reticulum; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.142; Membrane protein, integral; Transferase Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane Molecular Function:mannosyltransferase activity; chitobiosyldiphosphodolichol beta-mannosyltransferase activity Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; lipopolysaccharide biosynthetic process; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification Disease: Congenital Disorder Of Glycosylation, Type Ik |
| NCBI Summary: | The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik. [provided by RefSeq, Dec 2008] |
| UniProt Code: | Q9BT22 |
| NCBI GenInfo Identifier: | 73921663 |
| NCBI Gene ID: | 56052 |
| NCBI Accession: | Q9BT22.2 |
| UniProt Related Accession: | Q9BT22 |
| Molecular Weight: | Observed: 53 kDaPredicted: 53 kDa |
| NCBI Full Name: | Chitobiosyldiphosphodolichol beta-mannosyltransferase |
| NCBI Synonym Full Names: | ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase |
| NCBI Official Symbol: | ALG1 |
| NCBI Official Synonym Symbols: | HMT1; MT-1; CDG1K; HMAT1; HMT-1; Mat-1; hMat-1 |
| NCBI Protein Information: | chitobiosyldiphosphodolichol beta-mannosyltransferase |
| UniProt Protein Name: | Chitobiosyldiphosphodolichol beta-mannosyltransferase |
| UniProt Synonym Protein Names: | Asparagine-linked glycosylation protein 1 homolog; Beta-1,4-mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; GDP-mannose-dolichol diphosphochitobiose mannosyltransferase; Mannosyltransferase-1; MT-1; hMat-1 |
| UniProt Gene Name: | ALG1 |
| UniProt Entry Name: | ALG1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ALG1 antibody (CAB7818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of A549 cells using ALG1 antibody (CAB7818). Blue: DAPI for nuclear staining. |
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