Anti-AK1 Antibody (CAB1218)
- SKU:
- CAB1218
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-AK1 Antibody |
| 抗体コード: | CAB1218 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Mouse heart, Mouse skeletal muscle |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEEK LKKT KIIF VVGG PGSG KGTQ CEKI VQKY GYTH LSTG DLLR SEVS SGSA RGKK LSEI MEKG QLVP LETV LDML RDAM VAKV NTSK GFLI DGYP REVQ QGEE FERR IGQP TLLL YVDA GPET MTQR LLKR GETS GRVD DNEE TIKK RLET YYKA TEPV IAFY EKRG IVRK VNAE GSVD SVFS QVCT HLDA LK |
| 遺伝子ID: | 203 |
| Uniprot: | P00568 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 21kDa |
| 観察された分子量: | 22kDa |
| 同義語: | AK1, HTL-S-58j |
| バックグラウンド: | This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | AK1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency (HAAKD). Belongs to the adenylate kinase family. |
| UniProt Protein Details: | Protein type:EC 2.7.4.6; Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.3 Chromosomal Location of Human Ortholog: 9q34.1 Cellular Component: cytoplasm; cytosol Molecular Function:adenylate kinase activity; nucleoside diphosphate kinase activity Biological Process: nucleobase, nucleoside and nucleotide interconversion; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process Disease: Adenylate Kinase Deficiency, Hemolytic Anemia Due To |
| NCBI Summary: | This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
| UniProt Code: | P00568 |
| NCBI GenInfo Identifier: | 20178288 |
| NCBI Gene ID: | 203 |
| NCBI Accession: | P00568.3 |
| UniProt Secondary Accession: | P00568,Q9BVK9, Q9UQC7, |
| UniProt Related Accession: | P00568 |
| Molecular Weight: | 21,635 Da |
| NCBI Full Name: | Adenylate kinase isoenzyme 1 |
| NCBI Synonym Full Names: | adenylate kinase 1 |
| NCBI Official Symbol: | AK1 |
| NCBI Official Synonym Symbols: | HTL-S-58j |
| NCBI Protein Information: | adenylate kinase isoenzyme 1 |
| UniProt Protein Name: | Adenylate kinase isoenzyme 1 |
| UniProt Synonym Protein Names: | ATP-AMP transphosphorylase 1 |
| Protein Family: | Adenylate kinase |
| UniProt Gene Name: | AK1 |
| UniProt Entry Name: | KAD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AK1 antibody (CAB1218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using AK1 antibody (CAB1218) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using AK1 antibody (CAB1218) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using AK1 antibody (CAB1218). Blue: DAPI for nuclear staining. |
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