Anti-AGAP2 Antibody (CAB2759)
- SKU:
- CAB2759
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-AGAP2 Antibody |
| 抗体コード: | CAB2759 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 627-836 of human AGAP2 (NP_055585.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Jurkat, A-549, Raji, Mouse brain, Mouse lung, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 627-836 of human AGAP2 (NP_055585.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VRSL DLDD WPRE LTLV LTAI GNDT ANRV WESD TRGR AKPS RDSS REER ESWI RAKY EQLL FLAP LSTS EEPL GRQL WAAV QAQD VATV LLLL AHAR HGPL DTSV EDPQ LRSP LHLA AELA HVVI TQLL LWYG ADVA ARDA QGRT ALFY ARQA GSQL CADI LLQH GCPG EGGS AATT PSAA TTPS ITAT PSPR RRSS AASV GRAD APVA LV |
| 遺伝子ID: | 116986 |
| Uniprot: | Q99490 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 90kDa/124kDa |
| 観察された分子量: | 110kDa |
| 同義語: | AGAP2, CENTG1, GGAP2, PIKE |
| バックグラウンド: | The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| UniProt Protein Function: | CENTG1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol. |
| UniProt Protein Details: | Protein type:GAPs; Motility/polarity/chemotaxis; Tumor suppressor; GAPs, ARF Chromosomal Location of Human Ortholog: 12q14.1 Cellular Component: membrane; mitochondrion; nucleolus; nucleus Molecular Function:protein binding Biological Process: negative regulation of neuron apoptosis; negative regulation of protein catabolic process |
| NCBI Summary: | The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q99490 |
| NCBI GenInfo Identifier: | 97535883 |
| NCBI Gene ID: | 116986 |
| NCBI Accession: | Q99490.2 |
| UniProt Secondary Accession: | Q99490,O00578, Q548E0, Q8IWU3, A8K9F7, |
| UniProt Related Accession: | Q99490 |
| Molecular Weight: | 90,537 Da |
| NCBI Full Name: | Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 |
| NCBI Synonym Full Names: | ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 |
| NCBI Official Symbol: | AGAP2 |
| NCBI Official Synonym Symbols: | PIKE; GGAP2; CENTG1 |
| NCBI Protein Information: | arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 |
| UniProt Protein Name: | Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 |
| UniProt Synonym Protein Names: | Centaurin-gamma-1; Cnt-g1; GTP-binding and GTPase-activating protein 2; GGAP2; Phosphatidylinositol 3-kinase enhancer; PIKE |
| Protein Family: | Arf-GAP with GTPase, ANK repeat and PH domain-containing protein |
| UniProt Gene Name: | AGAP2 |
| UniProt Entry Name: | AGAP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AGAP2 antibody (CAB2759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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