Anti-AGA Antibody (CAB6352)
- SKU:
- CAB6352
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-AGA Antibody |
| 抗体コード: | CAB6352 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 161-346 of human AGA (NP_000018.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HepG2 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 161-346 of human AGA (NP_000018.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RNCQ PNYW RNVI PDPS KYCG PYKP PGIL KQDI PIHK ETED DRGH DTIG MVVI HKTG HIAA GTST NGIK FKIH GRVG DSPI PGAG AYAD DTAG AAAA TGNG DILM RFLP SYQA VEYM RRGE DPTI ACQK VISR IQKH FPEF FGAV ICAN VTGS YGAA CNKL STFT QFSF MVYN SEKN QPTE EKVD CI |
| 遺伝子ID: | 175 |
| Uniprot: | P20933 |
| セルラーロケーション: | Lysosome |
| 計算された分子量: | 37kDa |
| 観察された分子量: | 37kDa |
| 同義語: | AGA, AGU, ASRG, GA |
| バックグラウンド: | This gene encodes a member of the N-terminal nucleophile (Ntn) hydrolase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta chains that comprise the mature enzyme. This enzyme is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. Mutations in this gene are associated with the lysosomal storage disease aspartylglycosaminuria that results in progressive neurodegeneration. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is subject to proteolytic processing. |
| UniProt Protein Function: | AGA: a catabolic enzyme, aspartylglucosaminidase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. The lysosomal storage disease aspartylglycosaminuria is caused by a deficiency in the AGA enzyme. |
| UniProt Protein Details: | Protein type:Glycan Metabolism - other glycan degradation; EC 3.5.1.26; Hydrolase Chromosomal Location of Human Ortholog: 4q34.3 Cellular Component: endoplasmic reticulum; lysosome Molecular Function:peptidase activity; N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity; protein self-association Biological Process: protein deglycosylation; protein maturation; proteolysis Disease: Aspartylglucosaminuria |
| NCBI Summary: | Aspartylglucosaminidase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. The lysosomal storage disease aspartylglycosaminuria is caused by a deficiency in the AGA enzyme. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jan 2010] |
| UniProt Code: | P20933 |
| NCBI GenInfo Identifier: | 288558804 |
| NCBI Gene ID: | 175 |
| NCBI Accession: | P20933.2 |
| UniProt Secondary Accession: | P20933,Q4W5Q2, Q6FHN6, Q9UCK6, Q9UCK7, Q9UCK8, B2R7H2 D3DP47, |
| UniProt Related Accession: | P20933 |
| Molecular Weight: | 346 |
| NCBI Full Name: | N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase |
| NCBI Synonym Full Names: | aspartylglucosaminidase |
| NCBI Official Symbol: | AGA |
| NCBI Official Synonym Symbols: | GA; AGU; ASRG |
| NCBI Protein Information: | N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase; N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase; glycosylasparaginase; aspartylglucosylamine deaspartylase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase |
| UniProt Protein Name: | N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase |
| UniProt Synonym Protein Names: | Aspartylglucosaminidase; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidaseCleaved into the following 2 chains:Glycosylasparaginase alpha chain; Glycosylasparaginase beta chain |
| Protein Family: | Agaricus bisporus lectin |
| UniProt Gene Name: | AGA |
| UniProt Entry Name: | ASPG_HUMAN |
 | Western blot analysis of extracts of HepG2 cells, using AGA antibody (CAB6352) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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