Anti-ADAR Antibody (RACO0426)
- SKU:
- RACO0426
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | ADAR Antibody |
| Product SKU: | RACO0426 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, IHC |
| 推奨される希釈: | IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human ADAR1 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assembly of viral particles. However, high levels of ADAR1 inhibit HDV replication. |
| シノニム: | Double-stranded RNA-specific adenosine deaminase (DRADA) (EC 3.5.4.37) (136 kDa double-stranded RNA-binding protein) (p136) (Interferon-inducible protein 4) (IFI-4) (K88DSRBP), ADAR, ADAR1 DSRAD G1P1 IFI4 |
 | IHC image of RACO0426 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| UniProt Protein Function: | ADAR: Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site- selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed. Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90. 5 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:EC 3.5.4.37; Hydrolase; Nucleolus; RNA processing; RNA-binding Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus Molecular Function:double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; metal ion binding Biological Process: positive regulation of viral genome replication; base conversion or substitution editing; in utero embryonic development; response to virus; cytokine and chemokine mediated signaling pathway; miRNA-mediated gene silencing, miRNA loading onto RISC; somatic diversification of immune receptors via somatic mutation; adenosine to inosine editing; osteoblast differentiation; negative regulation of viral genome replication; pre-microRNA processing; protein import into nucleus; mRNA modification; erythrocyte differentiation; innate immune response; hemopoietic progenitor cell differentiation; gene expression; protein export from nucleus; mRNA processing; defense response to virus; negative regulation of apoptosis Disease: Dyschromatosis Symmetrica Hereditaria; Aicardi-goutieres Syndrome 6 |
| NCBI Summary: | This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P55265 |
| NCBI GenInfo Identifier: | 70167113 |
| NCBI Gene ID: | 103 |
| NCBI Accession: | NP_001020278.1 |
| UniProt Secondary Accession: | P55265,O15223, O43859, O43860, Q9BYM3, Q9BYM4, B1AQQ9 B1AQR0, D3DV76, |
| UniProt Related Accession: | P55265 |
| Molecular Weight: | |
| NCBI Full Name: | double-stranded RNA-specific adenosine deaminase isoform d |
| NCBI Synonym Full Names: | adenosine deaminase, RNA-specific |
| NCBI Official Symbol: | ADAR |
| NCBI Official Synonym Symbols: | DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP |
| NCBI Protein Information: | double-stranded RNA-specific adenosine deaminase |
| UniProt Protein Name: | Double-stranded RNA-specific adenosine deaminase |
| UniProt Synonym Protein Names: | 136 kDa double-stranded RNA-binding protein; p136; Interferon-inducible protein 4; IFI-4; K88DSRBP |
| Protein Family: | Double-stranded RNA-specific editase |
| UniProt Gene Name: | ADAR |
| UniProt Entry Name: | DSRAD_HUMAN |