Anti-ADAM12 Antibody (CAB7940)
- SKU:
- CAB7940
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ADAM12 Antibody |
| 抗体コード: | CAB7940 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 589-738 of human ADAM12 (NP_067673.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Mouse thymus, Mouse fat, Mouse ovary, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 589-738 of human ADAM12 (NP_067673.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ASRP VIGT NAVS IETN IPLQ QGGR ILCR GTHV YLGD DMPD PGLV LAGT KCAD GKIC LNRQ CQNI SVFG VHEC AMQC HGRG VCNN RKNC HCEA HWAP PFCD KFGF GGST DSGP IRQA EARQ EAAE SNRE RGQG QEPV GSQE HAST ASLT LI |
| 遺伝子ID: | 8038 |
| Uniprot: | O43184 |
| セルラーロケーション: | Cell membrane, Secreted, Single-pass type I membrane protein |
| 計算された分子量: | 80kDa/99kDa |
| 観察された分子量: | 100kDa |
| 同義語: | ADAM12, ADAM12-OT1, CAR10, MCMP, MCMPMltna, MLTN, MLTNA |
| バックグラウンド: | This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. |
| UniProt Protein Function: | ADAM12: contains a disintegrin and metalloprotease (ADAM) domain 12, which is a member of the ADAM protein family. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion, and the differentiation of macrophage-derived giant cells (MGC) and osteoclasts. 2 alternative splicing isoforms have been described: a shorter secreted form and a longer membrane-bound form. The shorter form stimulates myogenesis. |
| UniProt Protein Details: | Protein type:Protease; Membrane protein, integral; EC 3.4.24.-; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10q26 Cellular Component: nucleoplasm; mitochondrion; integral to membrane; plasma membrane; extracellular region Molecular Function:protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; SH3 domain binding Biological Process: epidermal growth factor receptor signaling pathway; proteolysis; cell adhesion; myoblast fusion |
| NCBI Summary: | This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014] |
| UniProt Code: | O43184 |
| NCBI GenInfo Identifier: | 229463008 |
| NCBI Gene ID: | 8038 |
| NCBI Accession: | O43184.3 |
| UniProt Secondary Accession: | O43184,O60470, Q5JRP0, Q5JRP1, Q6P9E3, Q6UWB0, |
| UniProt Related Accession: | O43184 |
| Molecular Weight: | 909 |
| NCBI Full Name: | Disintegrin and metalloproteinase domain-containing protein 12 |
| NCBI Synonym Full Names: | ADAM metallopeptidase domain 12 |
| NCBI Official Symbol: | ADAM12 |
| NCBI Official Synonym Symbols: | MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1 |
| NCBI Protein Information: | disintegrin and metalloproteinase domain-containing protein 12; meltrin-alpha; metalloprotease-disintegrin 12 transmembrane |
| UniProt Protein Name: | Disintegrin and metalloproteinase domain-containing protein 12 |
| UniProt Synonym Protein Names: | Meltrin-alpha |
| Protein Family: | Disintegrin and metalloproteinase domain-containing protein |
| UniProt Gene Name: | ADAM12 |
| UniProt Entry Name: | ADA12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ADAM12 antibody (CAB7940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat heart using ADAM12 antibody (CAB7940) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using ADAM12 antibody (CAB7940) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using ADAM12 antibody (CAB7940) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using ADAM12 antibody (CAB7940) at dilution of 1:100. Blue: DAPI for nuclear staining. |