Anti-ACTR2 Antibody (CAB5734)
- SKU:
- CAB5734
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ACTR2 Antibody |
| 抗体コード: | CAB5734 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACTR2 (NP_005713.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | THP-1, 293T, HepG2, HeLa, C6, Mouse spleen, Mouse lung, Mouse brain, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACTR2 (NP_005713.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDSQ GRKV VVCD NGTG FVKC GYAG SNFP EHIF PALV GRPI IRST TKVG NIEI KDLM VGDE ASEL RSML EVNY PMEN GIVR NWDD MKHL WDYT FGPE KLNI DTRN CKIL LTEP PMNP TKNR EKIV EVMF ETYQ FSGV YVAI QAVL TLYA QGLL TGVV VDSG |
| 遺伝子ID: | 10097 |
| Uniprot: | P61160 |
| セルラーロケーション: | Cell projection, Cytoplasm, cytoskeleton |
| 計算された分子量: | 44kDa/45kDa |
| 観察された分子量: | 45kDa |
| 同義語: | ACTR2, ARP2 |
| バックグラウンド: | The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ARP2: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Belongs to the actin family. ARP2 subfamily. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal Chromosomal Location of Human Ortholog: 2p14 Cellular Component: Arp2/3 protein complex; focal adhesion; cell projection; membrane; cytoplasm; cytosol; actin cap; actin cytoskeleton Molecular Function:actin filament binding; structural constituent of cytoskeleton; ATP binding Biological Process: axon guidance; meiotic chromosome movement towards spindle pole; asymmetric cell division; ephrin receptor signaling pathway; establishment and/or maintenance of cell polarity; innate immune response; cilium biogenesis; cytokinesis after meiosis; cell motility; cytoplasmic transport; spindle localization |
| NCBI Summary: | The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61160 |
| NCBI GenInfo Identifier: | 47117648 |
| NCBI Gene ID: | 10097 |
| NCBI Accession: | P61160.1 |
| UniProt Secondary Accession: | P61160,O15142, Q96C82, B2RCP5, D6W5F4, E9PF41, |
| UniProt Related Accession: | P61160 |
| Molecular Weight: | 394 |
| NCBI Full Name: | Actin-related protein 2 |
| NCBI Synonym Full Names: | ARP2 actin-related protein 2 homolog (yeast) |
| NCBI Official Symbol: | ACTR2 |
| NCBI Official Synonym Symbols: | ARP2 |
| NCBI Protein Information: | actin-related protein 2; actin-like protein 2 |
| UniProt Protein Name: | Actin-related protein 2 |
| UniProt Synonym Protein Names: | Actin-like protein 2 |
| UniProt Gene Name: | ACTR2 |
| UniProt Entry Name: | ARP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACTR2 antibody (CAB5734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using ACTR2 antibody (CAB5734) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human uterine cancer using ACTR2 antibody (CAB5734) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 antibody (CAB5734) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using ACTR2 antibody (CAB5734). Blue: DAPI for nuclear staining. |
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